中文摘要：

目的应用RNA干扰（RNAi）技术靶向沉默胶质瘤内水通道蛋白4（AQP-4）的表达，观察其对胶质瘤源性脑水肿的治疗效果。方法构建靶向AQP-4的siRNA质粒，免疫荧光化学方法检测其对C6细胞AQP-4的沉默效果；建立sD大鼠颅内c6胶质瘤模型，分对照组、空载组、无义序列组和siRNA组；聚合酶链式反应和蛋白印迹方法分别检测第3、6、9、12天AQP-4的mRNA和蛋白水平；干／湿比重法和Evans蓝测定法检测不同时相脑组织含水量和血脑屏障通透性改变；比较动物生存期。结果siRNA可沉默AQP-4表达；siRNA组脑组织水含量和血脑屏障通透性均明显低于对照组、空载组和无义序列组，动物生存期最长。结论靶向AQP-4的RNAi技术可在-定程度上减轻胶质瘤性脑水肿的发生和发展，为胶质瘤脑水肿提供了-种新的治疗策略选择。

英文摘要：

Objective To observe the treatment effect on glioma cerebral edema by RNA interference （ RNAi ） technology silencing aquaporin 4 （ AQP - 4 ） expression. Methods The siRNA plasmid targeting AQP -4 was constructed, and transfected into C6 glioma cells by liposome. Immunofluorescence assay was used to verify the silencing effect of AQP -4. The intracal C6 glioma model was established in SD rats, and four sub - groups, control group, empty vector transfected group, nonsense group and AQP -4 siRNA treated group, were divided. The AQP -4 mRNA and protein levels, in rats model brain tissue on 3, 6, 9, and 12 day, were detected by RT - PCR and Western Blot. Moreover, dry/ wet weight method and evans blue assay were used to detect the brain water content and blood - brain barrier permeability changes. Then the animal survival was compared. Results siRNA can effectively reduce AQP -4 expression in C6 glioma cells. Compared with the control and empty vector transfected group in vivo, AQP- 4 siRNA treatment group can effectively reduce AQP- 4 mRNA and protein level, decrease brain water content, and reduce blood - brain barrier permeability. The lifespans of the animals were prolonged. Conclusion The RNAi technology targeting AQP -4 can suppress the development of glioma brain edema to some extent, as well as provide a new therapeutic strategy to treat glioma brain edema.