中文摘要：

目的研究叶酸／聚酰胺-胺（FA／PAMAM）作为miR-7载体治疗胶质瘤U251的效果。方法将FA／PAMAM／miR-7络合物转染胶质瘤细胞株U251，绘制细胞生长曲线，检测细胞周期和细胞凋亡情况，Transwell体系检测瘤细胞迁移能力，软琼脂克隆实验检测细胞致瘤性；qRT—PCR方法检测miR-7水平，Western blot方法检测EGFR、P13K和AKT2的蛋白表达。结果获得稳定转染的U251细胞增殖速度减慢，细胞周期延长，凋亡细胞比例增多，细胞迁移能力、软琼脂克隆形成能力均有明显降低（P〈0．05）；miR-7水平明显提高，EGFR、P13K和AKT2蛋白水平均有一定程度降低（P〈0．05）。结论FA／PAMAM能够高效投递miR-7基因至胶质瘤细胞，发挥肿瘤生长抑制作用。

英文摘要：

Objective To explore the gene therapy effect of miR -7 mediated by folie acid /polyamide - amine vector on human U251 glioma. Method After transfeeting FA/PAMAM/miR - 7 gene into U251 glioma cell line, the cell growth curves were drawn, the cell cycle was detected, and apoptosis was detected by apoptosis in situ assay. Then the transwell system was utilized to explore the migration ability of tumor cells, and soft agar colony assay to examine the tumorigenicity. The quantitative RT - PCR was used to detect the miR -7 level, and western blot method was used to test the protein expression of EGFR,PI3K and AKT2. Results The cell proliferation rate slowed down, as well as theirs proportion reduction in S phase cells, and the ratio of apoptosis. The cell migration and soft agar colony forming ability in U251 cells transfeeted by miR - 7 were significantly reduced than control group （ P 〈 0.05 ）. With the increase of the miR - 7 level, the protein level of EGFR, PI3K and AKT2 in FA/PAMAM/miR - 7 group were much lower than the control group （ P 〈 0. 05 ）. Conclusions FA/PAMAM can transfect effectively miR- 7 into glioma, play a role to inhibit glioma cells proliferation.