中文摘要：

目的 分析不同条件下应用血卟啉衍生物（HpD）的光动力学疗法（PDT）对体外培养大鼠C6胶质瘤细胞的作用。方法MTT法检测PDT后C6细胞的生存率：（1）不同HpD浓度组（0、2、5、10、20、30及40mg／L）C6细胞PDT（628nm、20mW／cm^2、照光5min）后的生存率；（2）C6细胞分别与不同浓度HpD（0、5、10、20、30及40mg／L）孵育不同时间（0．5、1、3、6、12及24h）行PDT（628nm，20mW／cm^2）后的生存率；（3）不同照光强度下（10、20及30mW／cm^2）PDT后C6细胞的生存率。结果不同条件下PDT治疗对C6细胞的作用不同：（1）当HpD浓度低（2mg／L）、HpD与细胞孵育时间短（〈3h）时不能对细胞产生抑制或杀伤效应；（2）PDT后c6细胞的生存率随着HpD浓度的升高而降低，随着细胞与HpD孵育时间的延长而降低（〉12h后各组间差异无统计学意义），随着照光强度的增强而下降（20与30mW／cm^2之间差异无统计学意义）。结论HpD介导的PDT对体外培养大鼠C6胶质瘤细胞的作用与HpD浓度、HpD与细胞孵育时间及照光强度等有关，在不同条件下可表现为促进肿瘤细胞生长或杀伤肿瘤细胞的作用。

英文摘要：

Objective To evaluate the effect of hepatoprophyfin derivative （HpD） mediated photodynamic therapy （PDT） on cultured C6 glioma cells. Methods Cell survival rate examination after HpD-PDT treatment ： 3-（ 4,5-dimethylthiazol-2-yl ） -2,5-diphenyltertrazolium bromide （MTr） assay was used to determine cell survival rate 24 hrs after irradiation by Lumacare-051 as follows： （ 1 ） C6 cells were irradiated at 628 nm, 20 mW/cm^2 for 5 rain after being incubated with different concentration of HpD （0, 2, 5, 10, 20, 30 and 40 mg/L） respectively; （2） Ceils were irradiated at 628 nm, 20 mW/cm^2 for 5 rain after being incubated with HpD for different time periods （0. 5, 1, 3, 6, 12 and 24 hrs） ; （3） After being incubated with HpD, Cells were irradiated at 628 nm for 5 min at different light output power （ 10, 20 and 30 mW/cm^2）. Cell survival rate was calculated as follows： Survival（ % ） = （mean O. D. value of treated cell/mean O.D. value of control cell） × 100%. Results The effect of HpD-PDT on C6 cells differed under different conditions： No cell death was seen in low HpD concentration （2 mg/L） or short time incubation （ 〈3 h） groups; Cell survival rate decreased as the concentraion of HpD, incubation time with HpD and light output power increased. Conclusions The effect of HpD-PDT differes under different conditions, which is correlated to the concentration of HpD, incubation time of C6 cells with HpD and also the light output power.