中文摘要：

目的探讨血卟啉衍生物介导的光动力学疗法对体外培养大鼠C6胶质瘤细胞的杀伤效应。方法①应用MTT法测定不同HpD浓度组（0，2，5，10，20，30及40mg／L）c6细胞PDT（628nm，20mW／cm^2，照光5min）后的生存率；②采用光镜及电镜观察PDT后C6细胞的形态学变化；③DNA琼脂糖凝胶电泳检测各组样本的DNA条带；④TUNEL法检测各组样本PDT治疗后的细胞凋亡阳性率。结果①HpD浓度分别为2、5、10、20、30、40mg／L各组样本的细胞生存率分别为：110．31±6．25％、74．63％±6．57％、41．74％±4．16％、36．43％±2．14％、31．94％±2．25％及30．25％±2．88％：②PDT后c6细胞（HpD浓度〉5rag／L时）出现细胞回缩等改变；透射电镜观察发现10mg／L HpD组c6细胞出现染色质边集、凋亡小体形成等改变；20mg／LHpD组c6细胞出现胞膜破裂崩解、细胞核肿胀等表现；③DNA琼脂糖凝胶电泳检测发现10mg／L及5mg／LHpD浓度组产生了明显的梯状条带；④TUNEL测定5mg／L及10mg／LHpD浓度组c6细胞凋亡阳性率分别为33％和63％；20mg／LHpD浓度组细胞凋亡阳性率为6％。结论当HpD浓度为2mg／L时PDT对c6胶质瘤细胞无杀伤效应；当HpD≥5mg／L时PDT治疗能够引起c6胶质瘤细胞出现细胞凋亡及坏死，较低浓度（5．10mg／L）时c6细胞以凋亡为主，较高浓度HpD（≥20mg／L）时c6细胞主要以坏死为主。

英文摘要：

Objective To evaluate the effect of hepatoprophyrin derivative（HpD） mediated photodynamic therapy （PDT） on cultured C6 glioma cells. Methods （1）C6 glioma cells were irradiated at 628 nm, 20 mW/cm2 for 5 min afrer being incubated with different concentration of HpD （0, 2, 5, 10, 20, 30 and 40 mg/L）respectively, then cell survival rate was examined by MTT; （2）Inverted phase contrast microscope and transmitted eletron microscope （TEM） was used to examine the structural characteristics of C6 glioma cells of each group; （3）DNA agarose gel electrophoresis （AGE） was used to determine the DNA band of C6 glioma cells after HpD- PDT; （4）Apoptosis of C6 glioma cells were detected by TUNEL assay in differernt groups. Results （1）The cell survival rate of C6 cells in different HpD groups（2,5,10,20 and 40 mg/L）are 110.31 ± 6.25% .74.63± 6.57% .41.74±4.16% .36.43 ±2.14% .31.94±2.25% and 30.25 ± 2.88% respectively; （2）Morphological changes such as cell shrinkage were found after HpD-PDT; TEM observation found most C6 cells appeared a typical apoptotic changes in 10 mg/L group, While in 20 mg/L group, most of the C6 glioma cells appeared as necrotic; （3）DNA Agarose gel electrophoresis： Typical DNA ladder was seen in the 5 and 10 mg/L groups; （4）TUNEL detection showed： Some rather typical apoptotic positive cells were observed in 5 and 10 mg,/L grops with a proportionate of 33% and 63% respectively. Conclusions HpD-PDT can induce HpD concentration-related apoptosis and necrosis of C6 glioma cells, with a dominant apoptosis at low concentraion and necrosis at high concentraion; but in the low HpD concentration （2 mg/L）group, PDT didn＇t kill the C6 cells but seemed to promote cell growth.