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坛紫菜抗高温品系的筛选
  • ISSN号:1000-0615
  • 期刊名称:水产学报
  • 时间:0
  • 页码:112-119
  • 语言:中文
  • 分类:Q949.292[生物学—植物学]
  • 作者机构:[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海高校水产养殖学E-研究院,上海200090
  • 相关基金:国家高科技研究发展计划(“八六三”计划)资助项目(2002AA603023,2006AA10A413);国家自然科学基金资助项目(30170734.30571443);上海市自然科学基金资助项目(05RZ14110);上海市重点学科建设项目(Y1101);上海市E-研究院水产养殖学科特聘研究员项目(E030090)
  • 相关项目:坛紫菜单性生殖机理和单性育种学研究
中文摘要:

从已获得的4种坛紫菜优质高产品系中,筛选出抗高温的优良品系YZ-3。在28℃和29℃下培养15d后,YZ-3品系的壳孢子成活率分别达73.2%和52.6%,分裂率分别为100%和82.8%;而对照组野生型(WT)品系的壳孢子成活率分别只有10.3%和4.6%,分裂率分别为89.7%和65.9%;YZ-3品系的壳孢子成活率和分裂率均远高于WT品系。把在常温(24℃)下培养35d的壳孢子苗,再分别培养在24、28和29℃作抗高温试验,培养25d后发现,YZ-3品系的幼苗平均体长分别增加了33.6、23和15倍,而WT品系的苗平均体长分别只增加了7.7、1.4和0.9倍;YZ-3品系的苗平均体长分别是WT品系的3.8、8.8和7.4倍。在28℃和29℃组,WT品系幼苗培养15d就大面积腐烂;而YZ-3品系幼苗培养25d也不腐烂。上述结果表明YZ-3品系是抗高温的。

英文摘要:

In this paper, it was verified that the improved strain ( YZ-3 ) of Porphyra haitanensis Chang et Zheng obtained previously in the lab is a high-temperature resistant strain. When the conchospores of the YZ-3 strains and the wild-type strain (WT) were cultured at 28 ℃ and 29 ℃, the survival rate of conchospores was 73.2% and 52.6% for YZ-3 strain, but 10.3 and 4.6% for WT strain, respectively. The division rate of conchospores at 28 ℃ and 29 ℃ was 100 and 82.8% for YZ-3 strain, and 89.7% and 65.9% for WT strain, respectively. Both survival and division rates of conchospores of YZ-3 strain were significantly higher than those of WT strain either in culture at 28 ℃ and 29 ℃. When the conchospore germlings of YZ-3 and WT strains which have been cultured at 24 ℃ for 35 days were cultured at 24 ℃, 28 ℃ and 29 ℃ for 25 days, the mean length of blades increased 33.6, 23 and 15 times for YZ-3 strain, and only 7.7, 1.4 and 0.9 times for WT strain compared with the original mean length, respectively. When the blades cultured at 24 ℃, 28 ℃ and 29 ℃, the mean length of the blades of YZ-3 strain was 3.8, 8.8 and 7.4 times as WT strain, respectively. When the blades were cultured at 28 ℃ and 29 ℃, the blades of WT strain began to decay after cultured for 15 days, however, the blades of YZ-3 strain did not decay even being cultured for 25 days. These results indicated that YZ-3 strain is strongly resistant to high temperature (28 ℃ and 29 ℃ ).

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期刊信息
  • 《水产学报》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国水产学会
  • 主编:黄硕琳
  • 地址:上海市军工路334号上海水产大学
  • 邮编:201306
  • 邮箱:jfc@shou.edu.cn
  • 电话:021-61900228
  • 国际标准刊号:ISSN:1000-0615
  • 国内统一刊号:ISSN:31-1283/S
  • 邮发代号:4-297
  • 获奖情况:
  • 曾先后三次获中国科协专项基金资助,获首届《CAJ-CD规范》执行优秀奖,获水产类期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:26128