从已获得的4种坛紫菜优质高产品系中,筛选出抗高温的优良品系YZ-3。在28℃和29℃下培养15d后,YZ-3品系的壳孢子成活率分别达73.2%和52.6%,分裂率分别为100%和82.8%;而对照组野生型(WT)品系的壳孢子成活率分别只有10.3%和4.6%,分裂率分别为89.7%和65.9%;YZ-3品系的壳孢子成活率和分裂率均远高于WT品系。把在常温(24℃)下培养35d的壳孢子苗,再分别培养在24、28和29℃作抗高温试验,培养25d后发现,YZ-3品系的幼苗平均体长分别增加了33.6、23和15倍,而WT品系的苗平均体长分别只增加了7.7、1.4和0.9倍;YZ-3品系的苗平均体长分别是WT品系的3.8、8.8和7.4倍。在28℃和29℃组,WT品系幼苗培养15d就大面积腐烂;而YZ-3品系幼苗培养25d也不腐烂。上述结果表明YZ-3品系是抗高温的。
In this paper, it was verified that the improved strain ( YZ-3 ) of Porphyra haitanensis Chang et Zheng obtained previously in the lab is a high-temperature resistant strain. When the conchospores of the YZ-3 strains and the wild-type strain (WT) were cultured at 28 ℃ and 29 ℃, the survival rate of conchospores was 73.2% and 52.6% for YZ-3 strain, but 10.3 and 4.6% for WT strain, respectively. The division rate of conchospores at 28 ℃ and 29 ℃ was 100 and 82.8% for YZ-3 strain, and 89.7% and 65.9% for WT strain, respectively. Both survival and division rates of conchospores of YZ-3 strain were significantly higher than those of WT strain either in culture at 28 ℃ and 29 ℃. When the conchospore germlings of YZ-3 and WT strains which have been cultured at 24 ℃ for 35 days were cultured at 24 ℃, 28 ℃ and 29 ℃ for 25 days, the mean length of blades increased 33.6, 23 and 15 times for YZ-3 strain, and only 7.7, 1.4 and 0.9 times for WT strain compared with the original mean length, respectively. When the blades cultured at 24 ℃, 28 ℃ and 29 ℃, the mean length of the blades of YZ-3 strain was 3.8, 8.8 and 7.4 times as WT strain, respectively. When the blades were cultured at 28 ℃ and 29 ℃, the blades of WT strain began to decay after cultured for 15 days, however, the blades of YZ-3 strain did not decay even being cultured for 25 days. These results indicated that YZ-3 strain is strongly resistant to high temperature (28 ℃ and 29 ℃ ).