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用人工海水培养坛紫菜的初步研究
  • ISSN号:1674-5566
  • 期刊名称:上海水产大学学报
  • 时间:0
  • 页码:186-189
  • 语言:中文
  • 分类:S968.4[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室、上海高校水产养殖学E-研究院,上海200090
  • 相关基金:国家“863”计划(2002AA603023);国家自然科学基金(30170734,30571443);上海市自然科学基金(05RZ14110);上海市E-研究院水产养殖学科特聘研究员项目(E030090);上海市重点学科建设项目(Y1101);上海水产大学校长基金资助(2001-01)
  • 相关项目:坛紫菜单性生殖机理和单性育种学研究
中文摘要:

用天然海水与海水晶配置成的人工海水按10:0,8:2,6:4,4:6,2:8,0:10的体积比配成6种不同的培养液,用它们培养野生型坛紫菜叶状体。培养30d后,叶状体的生长情况如下:在100%天然海水中培养的坛紫菜叶状体生长最快,其它组的生长快慢依次为:含20%〉40%〉60%〉80%的海水晶人工海水组。在100%海水晶人工海水组培养的叶状体5d后死亡。叶状体叶绿素α(Chl.α)含量在20%海水晶人工海水组中最高,其余各组的含量相差不大,但藻红蛋白(PE)和藻蓝蛋白(PC)含量均比100%天然海水组稍低。用上述6种培养液培养坛紫菜丝状体,结果表明在60%海水晶人工海水组中培养的自由丝状体鲜重增加最明显,生长情况最好,100%天然海水组增重最少,其余各组增重差异不明显。坛紫菜丝状体在100%海水晶人工海水中生长良好,这说明可以用海水晶人工海水来培养坛紫菜丝状体。

英文摘要:

The young gametophytic blades of the wild type in Porphyra haitanensis were cultured respectively in different culture solutions which were composed of nature seawater and artificial seawater in different proportions ( 10 :0, 8:2,6:4,4:6,2:8,0: 10), and being enriched with MES medium. After 30 days of culture, the growth of the blades was the fastest in 100% natural seawater, and the growth speed in the others was 20% 〉 40% 〉 60% 〉 80% of seawater crystal, respectively. However, the blades cultured in 100% seawater crystal died after 5 days. The content of chlorophyll a (Chl. a) was highest in the blades cultured in 20% seawater crystal, and no obvious differences in the content of Chl. a were found in the blades cultured in other culture solutions, while the contents of phycoerythrin and phycocyanin in the blades were a litter lower than those of the blades cultured in the 100% seawater. The free-living conchocelis of Porphyra haitanensis were cultured in the above six kinds of culture solutions. After five weeks of culture, the increase of the wet weight of the free-living conchocelis cultured in 60% seawater crystal was most significant, but least increase of wet weight appeared in conchocelis cultured in 100% natural seawater. The weight increase of conchocelis cultured in other culture solutions did not show significant differences. The free-living conchocelis of P. haitanensis grew well in 100% of seawater crystal, indicating that its conchocelis can be cultured in 100% of seawater crystal.

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期刊信息
  • 《上海海洋大学学报》
  • 北大核心期刊(2011版)
  • 主管单位:上海市教委
  • 主办单位:上海海洋大学
  • 主编:陈裕东
  • 地址:上海市临港新城沪城环路999号
  • 邮编:201306
  • 邮箱:xuebao@shfu.edu.cn
  • 电话:021-61900229
  • 国际标准刊号:ISSN:1674-5566
  • 国内统一刊号:ISSN:31-2024/S
  • 邮发代号:4-604
  • 获奖情况:
  • 曾获《CAJ-CD规范》执行优秀奖,水产类期刊二等奖,全国优秀农业期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:3383