用天然海水与海水晶配置成的人工海水按10:0,8:2,6:4,4:6,2:8,0:10的体积比配成6种不同的培养液,用它们培养野生型坛紫菜叶状体。培养30d后,叶状体的生长情况如下:在100%天然海水中培养的坛紫菜叶状体生长最快,其它组的生长快慢依次为:含20%〉40%〉60%〉80%的海水晶人工海水组。在100%海水晶人工海水组培养的叶状体5d后死亡。叶状体叶绿素α(Chl.α)含量在20%海水晶人工海水组中最高,其余各组的含量相差不大,但藻红蛋白(PE)和藻蓝蛋白(PC)含量均比100%天然海水组稍低。用上述6种培养液培养坛紫菜丝状体,结果表明在60%海水晶人工海水组中培养的自由丝状体鲜重增加最明显,生长情况最好,100%天然海水组增重最少,其余各组增重差异不明显。坛紫菜丝状体在100%海水晶人工海水中生长良好,这说明可以用海水晶人工海水来培养坛紫菜丝状体。
The young gametophytic blades of the wild type in Porphyra haitanensis were cultured respectively in different culture solutions which were composed of nature seawater and artificial seawater in different proportions ( 10 :0, 8:2,6:4,4:6,2:8,0: 10), and being enriched with MES medium. After 30 days of culture, the growth of the blades was the fastest in 100% natural seawater, and the growth speed in the others was 20% 〉 40% 〉 60% 〉 80% of seawater crystal, respectively. However, the blades cultured in 100% seawater crystal died after 5 days. The content of chlorophyll a (Chl. a) was highest in the blades cultured in 20% seawater crystal, and no obvious differences in the content of Chl. a were found in the blades cultured in other culture solutions, while the contents of phycoerythrin and phycocyanin in the blades were a litter lower than those of the blades cultured in the 100% seawater. The free-living conchocelis of Porphyra haitanensis were cultured in the above six kinds of culture solutions. After five weeks of culture, the increase of the wet weight of the free-living conchocelis cultured in 60% seawater crystal was most significant, but least increase of wet weight appeared in conchocelis cultured in 100% natural seawater. The weight increase of conchocelis cultured in other culture solutions did not show significant differences. The free-living conchocelis of P. haitanensis grew well in 100% of seawater crystal, indicating that its conchocelis can be cultured in 100% of seawater crystal.