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条斑紫菜(Porphyrayezoensis)选育品系壳孢子的放散量与耐高温性研究
  • ISSN号:0029-814X
  • 期刊名称:《海洋与湖沼》
  • 时间:0
  • 分类:Q789[生物学—分子生物学]
  • 作者机构:[1]上海海洋大学水产与生命学院,上海201306
  • 相关基金:国家高技术研究发展计划(863计划)资助项目,2006AA10A413号;国家自然科学基金资助项目,30571443号;上海市科委重点项目,10391901100号;上海市教委重点学科建设项目,J50701号;农业部公益性专项,200903030-C号.
中文摘要:

模拟生产培育条件,对条斑紫菜选育品系(T-17)和野生型品系(WT)的贝壳丝状体在不同温度下的壳孢子放散量及壳孢子萌发体的生长发育进行了研究。结果表明,在18、23、24和25℃下,连续放散16d的平均每壳的壳孢子量,T-17分别为8.6×10^6、3.4×10^6、5.4×10^5和0个,胛分别为8.0×10^6、2.8×10^6、4.9×10^5和0个,两个品系之间无显著差异;18℃二组的壳孢子萌发和生长均正常。将23℃下放散的壳孢子分别在三种高温条件下(23℃、24℃下培养3d再转入25℃、25℃)培养16d,壳孢子成活率,T-17分别为84.6%、59.7%和45.5%,而WT分别为33.0%、20.9%和17.1%,差异极显著。在三种高温条件下,胛的壳孢子萌发体均出现了畸形和烂苗,而T-17的壳孢子萌发体,23℃组发育正常,其余二组的发育也不正常,出现畸形。上述结果说明,F-17品系不仅具有与WT一样的壳孢子放散量,而且壳孢子和萌发体的耐高温能力均比胛强,有望在生产上得到应用。

英文摘要:

The releasing, high-temperature resistance, and germination of the conchospores from an improved strain (T-17) of Porphyra yezoensis Ueda were studied. The average numbers of the total conchospores obtained from T-17 per shell over a releasing period of 16 days were 8.6×10^6, 3.4×10^6, 5.4×10^5, and 0 at 18, 23, 24, and 25℃, whereas were 8.0×10^6, 2.8×10^6, 4.9×10^5, and 0 for WT, respectively, There was no significant difference in the total numbers of conchospores between these two strains (P〉0.05). The conchospores of both strains, being released at 18℃, all grew normally. When the conchospores released at 23℃, were cultured at three different high-temperatures (e.g. 23 ℃, 25℃ after a 3-day pre-culture at 24℃, or 25℃) for 16 days, the survival rates for T-17 were 84.6, 59.7, and 45.5%, whereas were 33.0, 20.9 and 17.1% for WT, respectively. The conchospore germlings of WT in these three culture conditions were abnormal in shape, and some rotted, while the conchospores of T-17 that were released and cultured at 23℃ grew into germlings in normal morphology, while under the other two culture conditions, the germlings grew irregularly. All the results indicate that the T-17 strain could release enough conchospores for cultivation, and its conchospores are tolerant to high-temperatures and Can germinate normally into blades at 23 ℃, suggesting that the strain may offer broad application in the nori industry.

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期刊信息
  • 《海洋与湖沼》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国海洋湖沼学会
  • 主编:孙松
  • 地址:青岛市南海路7号中科院海洋所内
  • 邮编:266071
  • 邮箱:
  • 电话:0532-82898753
  • 国际标准刊号:ISSN:0029-814X
  • 国内统一刊号:ISSN:37-1149/P
  • 邮发代号:2-421
  • 获奖情况:
  • 2000年中国科学院优秀期刊一等奖,2000年山东省科委、新闻出版黑署评估优秀级,中国期刊方阵“双百”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:21021