中文摘要：

目的：以Fos蛋白表达为指标,分别研究后肢去负荷（hindlimb unloading,HU）以及去负荷一定时间后双轴旋转刺激对大鼠前庭核复合体（vestibular nucleus complex,VNC）各亚核神经元活动的影响。方法：动物分为后肢去负荷（即HU）组、HU处理后双轴旋转组（HU-R）和对照组（即假运动刺激组）。应用免疫组织化学ABC法,检查各组动物VNC各核团内Fos蛋白表达,统计Fos阳性神经元数目。结果：后肢去负荷1d可刺激VNC吻侧部前庭内侧核（MVe）、前庭下核（SpVe）两亚核内Fos阳性神经元数目较对照组有显著增加;但是,随后肢去负荷时间延长,VNC各核Fos阳性神经元数趋向于回到对照水平。2h双轴旋转刺激HU1d大鼠后,VNC所有核团内Fos阳性神经元数目都显著性增加;大鼠HU7d后再行双轴旋转刺激,MVe和SpVe内Fos阳性神经元数目有显著性增加。结论：地面模拟失重可刺激大鼠VNC神经元活动,随时间延长,模拟失重动物出现适应现象,前庭神经元活动减弱;运动刺激前庭感受器后,模拟失重动物VNC神经元活动显著增强。

英文摘要：

Objective： With Fos protein as index, the present study aims to investigate the effects of hindlimb unloading（HU）and double rotation following HU, respectively, on neural activities in the individual vestibular nuclei of vestibular nucleus complex （VNC） of rat. Methods： The animals were divided into 3 groups： HU group, double rotation stimulation following HU group （HU-R）, and control group in which rat was placed near the rotating equipment. Avidin-biotin complex （ABC） method was used to detect the number of Fos-positive neurons in each group. Results： One day after HU treatment significantly increased the number of Fos-positive neurons in medial and inferior vestibular nuclei （MVe and SpVe, respectively） over control group （P0.05）. However, prolonged HU treatment tended to decrease the number of Fos expression neurons to normal level. Two hours of double rotation stimulation on 1-day-HU rats significantly increased the number of Fos-positive neurons in all subnuclei of VNC （P0.05）, while the same stimulation on 7-day-HU rats only significantly increase the number of Fos-positive neurons in MVe and SpVe. Conclusion： Simulated weightlessness of ground-based experiment activated the VNC neurons, and the neuronal activities were suppressed with prolonged HU, due to animals’ adaptation to HU; motion stimulation to vestibular end-organs of HU rats significantly increased the number of Fos-positive neurons in VNC compared with the corresponding HU rats.