中文摘要：

FISH技术是进行基因和重复DNA序列在染色体上可视化作图的精确有效方法，已广泛应用于特异核苷酸序列的物理作图、基因组或染色体的识别、DNA序列的定量分析以及着丝粒、染色质空间结构分析等方面。综述了FISH技术在芸薹属作物基因组研究中的重要进展，并讨论了其应用前景。

英文摘要：

Fluorescence in situ hybridization （FISH） is an effective and accurate method to visualize the localization of genes and repetitive DNA sequences on chromosomes. This technique has been widely used for physical mapping of unique nucleotide sequences on specific chromosome regions, for identifying the specific genomes and chromosomes, for mearuring the size of various DNA molecules, and for revealing the spatial organization of the centromere and heterochromatin or euchromatin of different chromosomes. The present review mainly described the recent advances in Brassica genome research by fluorescence in situ hybridization and discussed its future applications in Brassica crops.