中文摘要：

为创建大白菜—结球甘蓝易位系,以大白菜—结球甘蓝2号单体异附加系（AC_2）为试材,对其花蕾辐射后获得的M_2植株进行游离小孢子培养。利用与结球甘蓝2号染色体对应的26个InDel标记对小孢子植株进行鉴定,结合细胞学观察,确认获得了6株添加甘蓝2号染色体片段的大白菜易位系植株。通过对InDel标记的加密设计,明确了6个大白菜易位系植株中均含有甘蓝特异标记C09-4和C09-4-52,片段大小为788.3 kb。选择两个来自不同M2单株的大白菜易位系‘AT2-1’和‘AT2-2’进行自交、回交及与大白菜高代自交系杂交,利用结球甘蓝特异的InDel标记对其后代进行鉴定。结果表明,两个大白菜易位系的85份自交后代、82份回交后代及188份杂交后代中均含有甘蓝特异标记C09-4和C09-4-52,说明这些易位系中的甘蓝染色体片段能够稳定遗传。

英文摘要：

Inorder to create Chinese cabbage–Cabbage translocation lines,M_2 plants obtained through pollen radiation of Chinese cabbage–Cabbage monosomatic alien addition line AC_2,were carried out to isolated microspore culture. Six translocation lines with cabbage chromosome 2 fragment were identified using 26 specific InDel markers corresponding cabbage chromosome 2 and cytological observation. The exogenetic fragment for those translocation lines was further identified to contain cabbage specific InDel markers C09-4 and C09-4-52 and the fragment size was about 788.3 kb by additional InDel markers. Two translocation lines‘AT2-1＇and‘AT2-2＇from two M_2 plants were selfed,backcrossed with‘85-1＇,and hybridized with Chinese cabbage inbred lines‘14-28＇and‘14-36＇,and the progenies were detected using the cabbage specific InDel markers C09-4 and C09-4-52 of cabbage. The results showed that 85 selfing progenies,82 backcrossing progenies and 188 hybrids contained the two cabbage specific markers,which indicated that the cabbage chromosome fragment could be transmitted to the next generation stability.