中文摘要：

目的探讨局灶脑缺血再灌注大鼠不同时间点大脑皮质炎症反应及IKK-NBD多肽干预对抗炎症反应的机制。方法采用线栓法制备Spragne-Dawley（SD）大鼠局灶脑缺血再灌注模型。将大鼠分为假手术组、模型组及IKK．NBD组，再下设再灌注1d和7d2个时相点。IKK-NBD组通过侧脑室定位定量注入4μIKK．NBD。对各组进行Zea-Longa神经功能评分，HE染色观察病理变化，Westernblot与荧光定量PT-PCR分别检测缺血皮质区c-rel蛋白和IKappaBot（IKBct）mRNA表达，ELISA检测缺血区炎症因子IL-1B和IL-10的含量。结果①再灌注1、7d时，IKK-NBD对模型大鼠行为学改善明显；②再灌注1、7d时，模型组病理改变较IKK-NBD组明显；③胞核c-rel蛋白：再灌注1、7d时，模型组均高于假手术组（P〈0．05）；再灌注1d时，IKK-NBD组高于假手术组（P〈0．05）；再灌注1、7d时，IKK-NBD组低于模型组（P〈0．05，P〈0．01）；随着时间延长模型组和IKK-NBD组均降低（P〈0．01）；④IKBotmRNA：再灌注1、7d时，模型组和IKK-NBD组均高于假手术组（P〈0．05），7d较1d回落；再灌注1d时，IKK-NBD组高于模型组（P〈0．01）；⑤IL-1p：再灌注1、7d时，模型组与IKK-NBD组均明显高于假手术组（P〈0．01），浓度随时间降低；再灌注1d时，IKK-NBD组较模型组减少（P〈0．01）；IL-10：再灌注l、7d时，模型组与IKK-NBD组均明显高于假手术组（P〈0．01），浓度随时间降低；再灌注1、7d时，IKK-NBD组均高于模型组（P〈0．01）。结论IKK—NBD在局灶脑缺血再灌注损伤后早期可以通过上调IKBot限制c-rel入核，下调IL-1B，上调IL-10而减轻再灌注后炎症损伤；而脑缺血再灌注恢复期，少量c-rel可上调IL-10起到抗炎作用。

英文摘要：

Objective To investigate the cerebral cortex inflammation of SD rats model of focal cerebral ischemia/reperfusion and the underlying mechanism of IKK-NBD peptides＇ anti-inflammation effect. Methods The MCAO/R SD rats were randomly assigned to 3 groups, that is, sham operation group, model group and IKK-NBD group, and each group was further randomly divided into 2 subgroups with reperfusion 1 and 7 d after 2 hours＇ ischemia. IKK-NBD of 4 μL was injected into the right lateral ventricle in 2 h before model infliction. Neurobehavior was evaluated by Zea-Longa score. HE staining was used for pathological observation, Western blotting and quantitative RT-PCR for mRNA and protein expression levels of c-rel and IkappaBet （Is：Bet）, and ELISA for the contents of IL-β and IL-10 in the isehemic cortex. Results IKK- NBD improved the behavioral recovery and reduced edema and necrosis after 1 and 7 d of reperfusion. The protein expression of c-rel in the cell nucleus was significantly higher in the model group than in the sham operation group （P 〈 0.05）, but significantly lower in IKK-NBD group than model group in 1 and 7 d after reperfusion （P 〈 0. 05, P 〈 0. 01 ）, while, in 1 d after reperfusion, it was significantly higher in IKK-NBD group than the sham operation group （P 〈 0. 05 ）. It was continuously decreased in the IKK-NBD group and model group with the elapse of time （P 〈 0. 05 ）. Expression level of Is：Bet mRNA was significantly higher in the model and IKK- NBD groups than the sham operation group in 1 and 7 d after reperfusion （P 〈 0. 05 ）, with that in day 7 lower than in day 1, and it was higher in IKK-NBD group than in model group in 1 d after reperfusion （P 〈0. 01 ）.IL-1β and IL-IO were significantly higher in the model and IKK-NBD groups than the sham operation group in 1 and 7 d after reperfusion （P 〈 0. 01 ）, and continued to reduce with the time, but in the day 1, IL-1β was lower in the IKK-NBD group than in the model group （P 〈0. 01 ）, and IL-