中文摘要：

目的：探讨电针促进局灶脑缺血/再灌注后缺血海马区血管再生的机制。方法180只雄性SD大鼠随机分为假手术组、模型组、电针组、CXCR4特异性拮抗剂AMD3100药物组、AMD3100＋电针组。线栓法制备右侧局灶脑缺血/再灌注模型。取大鼠“百会”穴（ GV 20）及左侧“四关”穴（合谷LI 4/太冲LR 3）为电针穴位，刺激时间为30 min/d。采用逆转录聚合酶链反应法（ RT-PCR）检测各组缺血海马区SDF-1α、CXCR4 mRNA表达，免疫荧光双标法检测CD34＋VEGFR2＋EPCs源性血管的表达。结果与假手术组比较，模型组与电针组SDF-1α、CX-CR4 mRNA表达明显增高（P＜0.05），其中电针组各时间点相对模型组增高更为显著（P＜0.05）。 AMD3100＋电针组缺血海马SDF-1α、CXCR4 mRNA表达在再灌注后1 d时明显高于电针组（ P＜0.05），但后逐渐下降，7 d时明显低于电针组（ P＜0.01）。与模型组比较，电针组再灌注3 d、7 d海马CD34＋VEGFR2＋EPCs源性血管表达明显增多（ P＜0.05）。与电针组比较，AMD3100＋电针组再灌注后7 d CD34＋VEGFR2＋EPCs源性血管表达明显下降（ P＜0.01）。 CD34＋VEGFR2＋血管表达变化与SDF-1α的表达变化显著相关（R＝0.784，P＜0.01）。结论电针可通过上调局灶脑缺血/再灌注大鼠缺血海马区SDF-1α/CXCR4的表达，促进血管再生。

英文摘要：

Objective To explore the effect of electroacupuncture on CD 34 ＋VEGFR2 ＋endothelial progenitor cell （EPC）-derived vessels and stromal cell-derived factor-1α（SDF-1α）/CXCR4, and study its mechanism of promoting an-giogenesis in hippocampus after focal cerebral ischemia /reperfusion .Methods A total of 180 healthy male adult Sprague Dawley （SD） rats were randomly divided into sham operation （sham） group, model （I/R） group, electroacupuncture （I/RE） group, I/RE plus AMD3100 （A specific antagonist of CXCR4） group （I/REA） and AMD3100 （I/RA） group. The rats received filament occlusion of the right middle cerebral artery for 2 hours followed by reperfusion .Electroacupunc-ture was applied to “Baihui” （GV20）/“Siguan” （Hegu LI 4/Taichong LR 3） acupoints for 30 min, once a day.The mR-NA expression of SDF-1αand CXCR4 were detected by reverse transcription-polymerase chain reaction （ RT-PCR） .Double immunofluorescence was used to stain CD 34 ＋VEGFR2 ＋EPC-derived vessels.Results Compared with the sham group, the mRNA expressions of SDF-1αand CXCR4 were significantly upregulated in I/R and I/RE group （ P＆lt;0.05 ） , but that in I/RE group was more significantly increased than I/R group（P＆lt;0.05）.In addition, the mRNA expression of SDF-1αand CXCR4 were highly increased on day 1 in the I/REA group than that of I/RE group, but decreased than that of I/RE group on day 7 after reperfusion （P＆lt;0.01）.CD34 ＋VEGFR2 ＋EPCs-derived vessels were obviously increased on 3d and 7d in the I/RE group compared with that of the I/R group, and significantly decreased on 7d in the I/REA group compared with that of the I/RE group （ P＆lt;0.01） .Conclusions Electroacupuncture can effectively promote an-giogenesis through upregulating the expression of SDF-1αand CXCR4 in rat ischemic hippocampus after focal cerebral is-chemia/reperfusion.