中文摘要：

目的探讨内皮型一氧化氮合酶(e NOS)在电针干预下对局灶脑缺血/再灌注大鼠骨髓及外周血中内皮祖细胞(EPCs)数量变化的影响,及其促大鼠脑内血管再生的机制。方法线栓法制备大鼠局灶脑缺血/再灌注模型,针刺"百会"穴及左侧"四关"穴。100只SD雄性大鼠随机分为正常组(N组)、模型组(I/R组)、模型+电针组(I/RE组)、模型+电针+L-NAME组(I/REL组)。除N组外的各组局灶脑缺血1.5 h后分为再灌注1、2、7 d三个亚组,每个亚组10只大鼠。在各时间点采用流式细胞术检测外周血及骨髓中VEGFR2+EPCs数量,荧光定量PCR技术检测缺血皮质区VEGFR2 mRNA的表达,免疫组织化学法检测缺血皮质区VEGFR2阳性细胞表达及CD34标记的微血管计数。结果与I/R组比较,电针可明显提高骨髓及外周血中EPCs的数量(P<0.01,P<0.05),而I/REL组的VEGFR2+EPCs数量相比于I/RE组则显著降低(P<0.01)。各时间点I/RE组缺血皮质区VEGFR2阳性细胞表达,VEGFR2 mRNA表达及CD34微血管计数明显高于I/R组(P<0.01),而I/REL组与之比较则显著减少(P<0.01)。结论电针可动员局灶脑缺血/再灌注大鼠内源性EPCs促大鼠缺血脑区血管再生,该作用在e NOS被抑制后减弱,推测电针动员EPCs促血管再生的作用与e NOS的激活有关。

英文摘要：

Objective To explore the effect and mechanism of electroacupuncture on eNOS in mobilizing endothe -lial progenitor cells (EPCs) in rat bone marrow and peripheral blood to promote revascularization in focal cerebral ischemi -a/reperfusion rat.Methods A total of 100 healthy male adult Sprague-Dawley (SD) rats were randomly divided into nor-mal group ( N) , model group ( I/R) , electroacupuncture group ( I/RE) and I/RE plus L-NAME ( A specific antagonist of eNOS) group (I/REL), and were further divided into 1 d, 2 d and 7 d subgroups after reperfusion, 10 rats in each group, in addition to the N group .The rats received filament occlusion of the right middle cerebral artery for 1.5 hours followed by reperfusion .“Baihui” ( GV 20)/“Siguan” ( Hegu LI 4/Taichong LR 3) were selected as acupucture points .Flow cytome-ter was used to detect the percentage of EPCs in bone marrow and peripheral blood .The expression of VEGFR2 mRNA was tested by fluorescence quantitative PCR .Immunohistochemical staining was used to detect VEGFR 2 +cells and to stain the CD34 +microvessels.Results Compared with the I/R group, there was a significant up-regulation of the percentage of EPCs in bone marrow and peripheral blood by electroacupuncture (P<0.01,P<0.05), but decreased by the inhibitor of eNOS (P<0.01).Compared with the I/R group, the VEGFR2 +cells, expression of VEGFR2 mRNA and CD34 +mi-crovessels were significantly increased in the I/RE group ( P<0.01 ) , but decreased in the I/REL group ( P<0.01 ) . Conclusions Electroacupuncture can effectively mobilize EPCs to promote the revascularization in focal cerebral ischemia /reperfusion rat .This effect is attenuated by inhibitor of eNOS , suggesting that the activation of eNOS mediated by electroa-cupuncture may be related to mobilizing EPCs to promote the revascularization .