中文摘要：

目的观测电针大鼠双侧合谷穴（LI4）对局灶性脑缺血再灌注后脑内血管内皮生长因子受体一2（VEGFR-2）mRNA和脑源性神经营养因子（BDNF）蛋白表达的影响，探讨神经发生和血管生成问可能的相互联系和作用。方法线栓法制备大鼠局灶性脑缺血再灌注模型。90只大鼠随机分为对照组（11=6）、模型组（n=42）和电针组（n=42）。观察局灶性脑缺血1h后再灌注2h、12h、24h、3d、7d、14d、21d共7个时相点，每个时相点6只大鼠。采用反转录酶．聚合酶链锁反应（RT-PCR）法检测VEGFR，2mRNA的表达，免疫组化法检测BDNF蛋白的表达。结果再灌注后12h、24h，电针组VEGFR．2mRNA明显高于模型组（P〈O．01），3d也高于模型组（P〈O．05）。与模型组比较，电针组海马区BDNF蛋白在再灌注后2h开始增加（P〈0.05），24h达高峰（P〈0．01），3d后仍有显著性差异（P〈0．05），但呈现增加幅度降低的趋势。结论电针能促进脑缺血再灌注后VEGFR．2mRNA、BDNF蛋白的表达；神经血管因子可能是神经发生与血管生成联系与作用的分子学基础。

英文摘要：

Objective To observe the effect of electroacupuncture on the expression of vascular endothelial growth factor receptor-2 （VEGFR-2） mRNA and brain-derived neurotrophic factor （BDNF） protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Methods The focal cerebral ischemia/reperfusion model was established with filament occlusion. 90 rats were randomly divided into control group （n=6）, model group （n=42） and electroacupuncture group （n=42）. They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after isch- emia/reperfusion, 6 rats in each points. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of VEG- FR-2 mRNA. Immunohistology method was used to detect the expression of BDNF protein. Results The expression of VEGFR-2 mRNA was higher in the electroacupuncture group than in the model group 12 h, 24 h, 3 d after reperfusion （P〈0.05）. The expression of BDNF pro- tein in the hippocampus increased 2 h （P〈0.05）, reached peak 24 h （P〈0.01） and still higher 3 d （P〈0.05） in the electroacupuncture group than in the model group after reperfusion. Conclusion Electroacupuncture can upregulate the expression of VEGFR-2 mRNA and BDNF protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Neuro-angiogenic factor is possibly the molecular basis of coordinat- ed interaction of the nervous and vascular system.