中文摘要：

运用脂质体转染第X期胚盘细胞的方法将外源质粒pEGFP—C2经脂质体包裹后注射到鹌鹑种蛋X期胚盘下腔，孵化种蛋。提取孵化6d的鹌鹑胚基因组DNA，进行PCR检测，嵌合体阳性率为84％，证实外源基因在胚胎中的表达。对孵化至出壳的G0代鹌鹑组织进行DNA提取并PCR分析，以及组织切片荧光检测，证实外源基因在瓯代鹌鹑组织中成功表达。结果表明脂质体转染胚盘细胞是一种制备转基因鹌鹑行之有效的方法。

英文摘要：

In order to identify the availability of liposome transfection of foreign DNA as an effective way in making transgenic quail,foreign plasmid pEGFP-C2 was injected into the subgerminal cavity of the fresh fertilized eggs before incubation,and the embryos of 6 day-old were collected to identify the distribution of the exogenous DNA, The positive ratio was 84 %, the exogenous gene was expressed in embryos. Using Go generation quail tissue for DNA extraction and PCR analysis, and tissue sections for fluorescent detection,the successful expression of foreign gene in Go generation quail tissue was confirmed. This research results indicates the method is effective to produce transgenic quail.