中文摘要：

Osteochondral allografting 被证明了是一个有用方法对待 diseased 或损坏联合表面的区域。供应在获得和利用之间的一个缓冲区的接枝的运作的长期的股票将贡献这种技术的商品化或工业化。玻璃化被认为是为关节的软骨(交流) 的成功的保藏的一个有希望的方法，但是高集中 cryoprotectants (CPA ) 被使用它可以引起高细胞的毒性。当温度被降低时，减少 CPA 毒性的一个有效方法是逐渐地增加 CPA 集中。在严寒的温度理解 CPA 浸透的机制为设计 cryopreservation 协议是重要的。在这研究，在在严寒的温度的羊似的交流的 dimethyl sulfoxide (Me2SO ) 的浸透试验性地被学习。 Pretreated 交流磁盘在三个温度层次在不同时间( 0 ， 5 ， 15 ， 30 ， 50 ， 80 ，和 120 min )的 Me2SO 解决方案被暴露( 10 ， 20 ，吗并且 30 眠瑩??潣瑮湩潵獵挠牵敶漠?牡楢牴牡?楦獲?牯敤?楬敮牡攠汬灩楴?楤晦牥湥楴污漠数慲潴獲漠敶??潣灭捡?慭楮潦摬眠瑩?潢湵慤祲?湕敤?桴?摡楤楴湯污愠獳浵瑰潩?景琠敨眠慥?湩敮?湵煩敵挠湯楴畮瑡潩?牰灯牥祴?敷搠牥癩?桴?潣瑮湩極祴漠??敲慬整?畣癲?景? 污敤r 90 挠湡愠捣汥牥瑡?桴?楤晦獵潩?景挠牡潢?牦浯琠敨瀠楲牯愠獵整楮整朠慲湩戠畯摮牡?湩潴挠牡楢敤?潈辔?辔？？

英文摘要：

Osteochondral allografting has been proved to be a useful method to treat diseased or damaged areas of joint surfaces. Operational long-term stocks of grafts which supply a buffer between procurement and utilization would contribute to the commercialization or industrialization of this technology. Vitrification has been thought to be a promising method for successful preservation of articular cartilage （AC）, but high concentration cryoprotectants （CPAs） are used which may cause high cellular toxicity. An effective way to reduce CPA toxioity is to increase CPA concen- tration gradually while the temperature is lowered. Understanding the mechanism of （31~A permeation at subzero temperatures is important for designing the cryopreservation protocol. In this research, the permeation of dimethyl sulfoxide （MezSO） in ovine AC at subzero temperatures was studied experimentally. Pretreated AC discs were ex- posed in Me2SO solutions for different time （0, 5, 15, 30, 50, 80, and 120 min） at three temperature levels （-10, -20, and -30℃）. The Me2SO concentration within the tissue was determined by ultraviolet （UV） spectrophotometry. The diffusion coefficients were estimated to be 0.85×10-6, 0.48×10-8, and 0.27×10-6 cm2/s at -10, -20, and -,30℃, respectively, and the corresponding activation energy was 29.23 kJ/mol. Numerical simulation was performed to com- pare two Me2SO addition protocols, and the results demonstrated that the total loading duration could be effectively reduced with the knowledge of permeation kinetics.