中文摘要：

研究了修饰核苷7-甲基鸟苷（7-Methylguanosine）的电化学行为及测定方法.在pH 1.98的B-R缓冲液中,用循环伏安法（CV）,线性扫描伏安法（LSV）、微分脉冲伏安法（DPV）等现代电化学技术研究7-甲基鸟苷在玻碳电极（GCE）上的伏安行为.实验表明,7-甲基鸟苷在＋1.036 V（vs.SCE）电位处产生一个阳极氧化峰,峰电流与7-甲基鸟苷的浓度在5.0×10^-5～2.0×10^-6 mol/L范围内呈良好的线性关系,最低检测限（D=2σ/K）为4.1×10^-7 mol/L.并用恒电位库仑电解法等方法对其氧化机理进行了较为详细的探讨,得到了可能的电极反应机理：7-甲基鸟苷在玻碳电极上的电极反应是属于失1质子和2电子的不可逆的氧化反应.

英文摘要：

The electrochemical response of 7-methylguanosine in pH 1.98 B-R buffer was investigated by cyclic voltammetry （CV）, linear sweep voltammetry （LSV） and differential pulse voltammetry （DPV） method. The differential pules voltammetry exhibited an oxidation current peak of 7-methylguanosine at the potential of ＋1.036 V （vs. SCE）. The peak current is linearly increased with the concentration of 7- methylguanosine in the range of 5. 0× 10^-5-2. 0× 10^-6 mol/L. The detection limit is 4. 1 × 10^-7 mol/L. The possible mechanism for the irreversible reaction was assumed to be the oxidation lossing of one proton and two electrons.