中文摘要：

目的 探讨pAdBM5-mAFP-DC瘤苗对C57BL／6J小鼠移植性肝癌发生、发展的阻断作用。方法 40只C57BL／6J小鼠随机分为A、B、C、D、E组，每组8只。以免疫表达mAFP基因的重组腺病毒转染DC（pAdBM5-mAFP—DC）为A组；空pAdBM5质粒免疫为B组；以免疫表达mAFP基因的质粒DNA（pAdBM5-mAFP）为C组；单纯免疫DC为D组；以注射单纯PBS为对照组的E组。免疫方法：A组和D组在每只小鼠的右腋下注射5×10^5个细胞；B组和C组在每只小鼠的右腋下注射10．0μg质粒；E组仅注射0．1mlPBS。每周注射1次，连续免疫4次。在初次免疫后的第3周给所有小鼠移植Hepa1-6肝癌细胞。观察小鼠移植瘤的生长情况。于移植肿瘤第14天处死小鼠，观察成瘤情况，计算抑瘤率。结果 A组有3只小鼠未长出移植瘤。处死小鼠称瘤重，发现A组瘤重与其他各对照组瘤重比较差异有显著性（P〈0．05或0．01）。A、B、C、D各组肿瘤抑制率分别为：68．31％、13．23％、52．1％和38．54％，A组抑瘤率显著高于其它各组（P〈0．05或0．01）。结论 重组pAdBM5-mAFP—DC瘤苗免疫小鼠，可在一定程度上使小鼠获得免疫保护，降低肝癌的发生率。

英文摘要：

Objective To investigate the inhibitory effect of pAdBMS-mAFP-DC tumor vaccine on the transplanted liver cancer in mice. Methods 40 C57BL/6J mice were randomly divided into group A, B, C, D and E （8 mice in each group）. Group A was immunized with mAFP gene-modified DC （pAdBM5-mAFP-DC） ; Group B was immunized with simple pAdBM5 plasmid ; Group C was immunized with recombinant pAdBMS-mAFP plasmid ; Group D was immunized with simple DC ; Group E was injected with PBS. Each mouse in group A and D was immunized with 5 × 10^5 cells, in group B and C was immunized with 10. 0μg plasmid DNA, in group E was injected with 0.1ml PBS. They were immunized once a week for a consecutive 4 weeks. At the third week, all the mice were transplanted with Hepal-6 cells. All mice were killed 14 days after Hepa1-6 immunization. Results There was no tumorigenesis could be observed in 3 of 8 mice in group A. The average tumor weight of group A was far more lighter than that in the other groups, with the statistical significance （P 〈0. 05 or 0. 01 ）. The tumor-inhibited rate of group A, B, C and D were 68.31%, 13.23%, 52. 1% and 38.54%, respectively. The tumor-inhibited rate for group A was significantly higher than that in the other groups（ P 〈0. 05 or 0. 01 ）. Conclusion mAFP gene-modified DC tumor vaccine could induce immunoprotection and reduce the risk of tumorigenesis.