中文摘要：

目的对比研究肝癌患者和正常人外周血树突状细胞（Dc）的诱导及其功能异同，探讨能否从肝癌患者外周血诱导出功能正常、具有抗肿瘤作用的DC疫苗。方法取肝癌患者和正常人外周血分离DC前体细胞，应用rhGM—CSF、rhIL-4及肝癌细胞抗原诱导DC。观察DC生长过程的形态学变化，以流式细胞术检测DC表面分子；EUSA法检测DC培养上清中IL-12水平；3H—TdR掺入法检测肝癌细胞抗原致敏DC刺激T细胞增殖的能力；乳酸脱氢酶4小时释放法检测肝癌细胞抗原致敏DC诱导的细胞毒性T淋巴细胞（CTL）的杀瘤活性。结果从肝癌患者和正常人外周血DC前体细胞诱导的DC具有相似的生长过程和细胞形态及功能，其表面分子HLA—DR、CD1a、CD83、CD80、CD86、ICAM-1的表达水平、DC培养上清中IL-12水平、DC刺激T细胞增殖的能力以及所诱生的CTL杀瘤活性等方面比较，差异均无统计学意义（P〉0．05）。结论可从肝癌患者外周血诱导出功能正常并具有抗肿瘤作用的DC瘤苗，为以DC基础的肝癌的免疫治疗提供实验依据。

英文摘要：

Objective To investigate the potent anti-tumor activity of induced dendritic cells （DC）derived from the peripheral blood of patients with hepatocellular carcinoma（HCC）,and to lay the foundation for a DC vaccine against HCC. Methods DC were isolated from the peripheral blood of HCC patients and healthy individuals and induced in vitro using rhGM-CSF,rhlL-4 and HCC antigen. The morphology of DC was observed by phase contrast microscopy, and surface molecule expression was analyzed by fluorescence-activated cell sorting.IL-12 released by DC into the culture medium was detected by ELISA.T cell proliferation was quantitated by 3H- TdR uptake,and cytotoxic of T-lymphocyte （CTL）activity was assessed using the LDH release assay. Results Comparison of induced DC from HCC patients and induced DC from healthy individuals revealed no significant differences in growth characteristics ;expres- sion levels of surface molecules such as HLA-DR,CD1a,CD83,CD80,CD86,ICAM-1,and IL-12;and ability to stimulate T lymphocytes and induce CTL activity（P〉0.05 ）. Conclusions Induced DC from HCC patients can exert anti-tumor effects similar to those observed with induced DC from healthy individuals.These results provide an experimental basis for developing HCC immunotherapies involving DC.