中文摘要：

目的：研究人肝癌组织中浸润树突状细胞（Dc）的数量和表面分子表达的临床意义。方法：原发性肝细胞癌（肝癌）术后组织标本30例，采用免疫组化方法（即用型两步法Supervision^TM）检测肝癌组织中DC标志蛋白S-100的表达；从肝癌组织中分离浸润性单个核细胞，流式细胞术检测DC的表面分子表达，并用RT-PCR技术检测肝癌组织IL-10、TGF-β1和VEGF-AmRNA。结果：肝癌组织中DC的数量少，高分化肝癌组织中DC浸润数量[（27．22±15．38）个／HPF]高于低分化肝癌[（11．92±8．56）个／HPF]（P〈0．01）；肝癌组织中浸润DC的表面分子如CDla、HLA-DR、CD80、CD86和CD54等MHC-Ⅰ、Ⅱ类分子，共刺激分子和黏附分子存在低表达或不表达，与肝癌的分化程度密切相关。在mRNA水平上，IL-10、TGF-β1和VEGF-A基因在肝癌组织中的表达显著高于正常肝组织（P〈0．001，P〈0．05）。结论：肝癌组织中浸润DC的数量减少并存在功能缺陷，与肝癌分化程度密切相关。

英文摘要：

Objective： To investigate the clinical significance of dendritic cells infiltration and surface molecules in human hepatocellular carcinoma （HCC）. Methods： 30 cases of HCC were studied with immunohistochemical staining , surface molecules of infiltrating DC were examined by methods including cells isolation and FACS. Reverse transcript-polymerase chain reaction （RT-PCR） was used to detect IL-10, TGF-β1 and VEGF-A mRNA expression in HCC tissue. Result： The number of infiltrating DC in HCC decreased, and the number of DC infiltration in the group with good differentiationE（27.22±15.38）个/HPF] was significantly higher than that in the group with poor differentiation [（11.92±8.56）个/HPF] （ P 〈0.01）. The levels of some surface molecules such as MHC class Ⅰ （CDla） and Ⅱ （HLA-DR）, and constimulating molecules （CD80, CD86）, and adhesion molecules（CD54） in HCC were down-regulated or negative. In the mRNA level,the expressions of IL-10, TGF-β1 and VEGF-A gene in HCC tissue were higher than those of normal liver tissue（ P 〈0. 001, P 〈0.05）. Conclusion： The infiltrating DC of HCC decreased, and it was a kind of dysfunction DC and was closely linked to differentiation degree of HCC.