中文摘要：

为研究液泡H＋-ATPase c亚基VHA-c4和VHA-c5基因在植物生长发育过程中的作用,本研究构建了拟南芥VHA-c4和VHA-c5过表达载体并转化野生型拟南芥,分别获得9个和7个T2代转基因纯合体株系。采用半定量RT-PCR方法对过表达VHA-c4和VHA-c5的转基因纯合体进行阳性鉴定,发现其mRNA表达量均高于对照。对转基因纯合体进行暗培养和正常光照培养,结果显示,黑暗条件下,所有VHA-c4转基因株系的主根变短,而在正常光照下,所有VHA-c5转基因株系的主根变短,推测VHA-c4和VHA-c5分别在黑暗和光照条件下影响植物根的生长。用ABA和糖（葡萄糖和蔗糖）处理转基因纯合体,结果显示它们与野生型的表型无明显差异,表明VHA-c4和VHA-c5基因的过表达没有影响拟南芥对ABA和糖的响应。

英文摘要：

To study the role of vacuole H~＋-ATPase subunit c genes on plant growth and development,over-expression vectors of VHA-c4 and VHA-c5 from Arabidopsis thaliana were constructed and introduced into wild-type A. thaliana,and 9 and 7 homozygous T2 generations of VHA-c4 and VHA-c5 overexpressed transgenic lines were obtained,respectively. Semi-quantitative RT-PCR methods were used for identification of the positive VHA-c4 and VHA-c5 transgenic homozygotes,and their corresponding transcript levels were increased in every transgenic line. When cultivated under different light conditions,the VHA-c4 transgenic plants exhibited a reduction in primary root length compared with that of the wild-type when cultivated in the dark,whereas the VHA-c5 transgenic plants showed reduced primary root length under normal light conditions. These results indicated that VHA-c4 and VHA-c5 affected the growth of roots during light / dark cycles. Following ABA and sugar（glucose and sucrose） treatment,no significant differences were found between the transgenic homozygote phenotype and wildtype,indicating that VHA-c4 and VHA-c5 overexpression did not affect the response of Arabidopsis to ABA or sugar.