中文摘要：

VHA-c3基因是拟南芥液泡H^＋-ATPasec亚基的5个同源基因之一，已有研究结果表明其与植物抗非生物胁迫有关。在克隆不同长度VHA-c3基因上游调控序列的基础上，利用GUS报告基因，研究了VHA-c3基因的植物组织及器官定位。结果表明：VHA-c3起始密码子上游772bp的序列内存在着VHA-c3基因的基本启动子元件，可指导基因组成型表达在拟南芥的叶片、表皮毛、叶柄、根、雄蕊、柱头和萼片；在VHA-c3起始密码子ATG上游2812bp～2234bp片段和1496bp～772bp片段中各存在一个负调控元件，在2234bp～1496bp片段中存在一个正调控元件，它们的存在可控制基因在气孔中的表达。

英文摘要：

VHA-c3 gene is one of the five homologous genes which belong to the subunits of vacuole H^＋ - ATPase in Arabidopsis thaliana. Former reports indicated that it is related with resistance to abiotie stress in plants. Different fragments of the upstream regulation sequences of Arabidopsis VHA-c3 gene were cloned, and fused with GUS reporter gene, to study the localization of VHA-c3 gene. The essential element for the basal activity of VHA-c3 is located within the -722bp fragments upstream of the translation start eodon of VHA-c3, it can guide genes＂ constitutive expression in leaf blade, triehome, petiole, root, stamen, stigma and sepal in Arabidopsis; And there is two negative regulatory elements located in -2812bp to -2234bp and -1496bp to -772bp respectively, and an up-regulating eis-element from -2234bp to -1496bp fragment of VHA-c3 gene, which may control the gene＇s expression in guard cell.