中文摘要：

为初步探讨液泡H^＋-ATPase c亚基基因（VHA-c1）在植物生长发育及信号转导中的作用,该实验构建了拟南芥VHA-c1的过表达载体,转化并获得转基因拟南芥纯合株系,通过半定量RT-PCR技术分析了VHA-c1的表达量,然后对其进行暗培养、ABA和糖处理。结果显示：（1）该实验获得7个T2代转基因纯系,其mRNA表达水平均高于野生型,表明过表达载体的构建是有效的。（2）黑暗条件下,6个拟南芥VHA-c1过表达纯合株系的根长变短。（3）光照条件下,4个转基因株系主根伸长和子叶的展开以及7个转基因株系的种子萌发对ABA的抑制不敏感。（4）分别有5个和4个转基因株系的种子萌发对葡萄糖和蔗糖的抑制不敏感。推测VHA-c1可能参与了ABA和糖介导的信号转导途径,并可能影响了拟南芥根细胞的扩展。

英文摘要：

For preliminary study the function of vacuole H^＋-ATPase subunit c gene （VHA-cl） in plant growth and signal transduction, we constructed an over-expression vector of VHA-c 1 in Arabidopsis thaliana and introduced it into wild-type A. thaliana. The expression levels of VHA-c1 were analyzed by semiquantitative reverse transcription-polymerase chain reaction （RT-PCR）, then the VHA-c1 transgenic homozygotes were treated by dark condition,ABA or sugar. The results showed that： （1）7 lines of VHA-c1 tansgenic homozygotes were obtained,the corresponding transcript level of them increased,indicating that the overexpression constructs are effective. （2）When grown under dark condition,the root length in 6 VHA-c 1 tansgenic homozygotes lines were reduced. （3）With treatment by ABA, 4 lines reduced the sensitivity in taproot growth and the extent of cotyledon unfolding; 7 lines reduced the sensitivity in seed germination. （4）5 lines and 4 lines also reduced the sensitivity to inhibition on seed germination by glucose and sucrose,respective. This indicates that the vacuolar VHA-c1 might be involved in the signalling pathway mediated by ABA and sugar,and possible effect on cell expension.