中文摘要：

波伊昂·莱克是在中国的最大的内陆淡水湖并且包含野生的鸟和水鸟的许多种类。我们在 1 月期间在波伊昂·莱克在九个半人工的水鸟农场进行了鸟的流行性感冒病毒的调查到 2010 的三月。从收集的 1036 个 cloacal 拖把，三 H3N2 和一个 H4N6 流行性感冒病毒从健康野鸭被孤立。所有 isolates 遗传上并且种系发生地被描绘。分析通常认为哈劈开地点证明四孤立的所有拥有了分子的特征(为 H3N2 病毒的 QTRGL，为 H4N6 病毒的 PEKASR ) 低层的病原的鸟的流行性感冒(LPAI ) 病毒。病毒的染色体的种系发生的分析证明四个病毒孤立的所有在流行性感冒病毒的欧亚的 clade 聚类。病毒的 M 基因与高度病原的 H5N1 流行性感冒病毒拥有了最高的相同。另外，在一样的农场的 H3N2 和 H4N6 的合作感染被观察。并且有趣地，我们从一样的农场， H4N6 的 PB1 和 PB2 基因片断 H3N2 紧张在代替了那些与染色体分类的证据孤立二个子类型病毒(H3N2 和 H4N6 ) 和他们的子孙病毒(H3N2 ) 。动物感染实验的结果证明所有四个孤立的病毒是低层的对鸡病原、对老鼠不病原，它与基因分析的结果一致。

英文摘要：

Poyang Lake is the largest inland freshwater lake in China and contains many species of wild birds and waterfowls. We conducted a survey of avian influenza viruses in nine semi-artificial waterfowl farms in Poyang Lake during January to March of 2010. Out of 1036 cloacal swabs collected, three H3N2 and one H4N6 influenza viruses were isolated from healthy mallards. All the isolates were genetically and phylogenetically characterized. The analysis of putative HA cleavage sites showed that all the four isolates possessed the molecular characteristics （QTRGL for H3N2 viruses, PEKASR for H4N6 virus） of lowly pathogenic avian influenza （LPAI） virus. The phylogenetic analysis of the viral genomes showed that all four virus isolates clustered in the Eura- sian clade of influenza viruses. The M gene of the viruses possessed the highest homology with highly pathogenic H5N1 influenza viruses. In addition, co-infection of H3N2 and H4N6 in the same farm was observed. And interestingly, we isolated two subtypes viruses （H3N2 and H4N6） and their progeny virus （H3N2） with evidence of genome reassortment from the same farm, in which the PBI and PB2 gene segments of H4N6 replaced those of the H3N2 strain. The results of animal infection experiments showed that all the four isolated viruses were lowly pathogenic to chickens and not pathogenic to mice, which was consistent with the results of genetic analysis.