中文摘要：

胚胎干细胞（ES细胞）能够不断地进行自我更新来维持其多能性,很多转录因子共同调控着ES细胞的自我更新和多能性,Nanog就是其中之一,然而Nanog维持ES细胞多能性的机制并不清楚。p300是真核生物中普遍存在的转录辅助因子,与许多转录因子共同作用调控下游基因的表达。为探索p300是不是能够影响Nanog的转录活性,我们在细胞中共转Nanog（或突变体）及报告基因和p300,结果表明p300能够通过homeobox结构域增强Nanog的转录激活活性。

英文摘要：

Embryonic stem cells（ES cells） undergo self-renewal to maintain their pluripotency.The self-renewal and pluripotency of ES cells are under tight control of many transcription factors.Nanog is one of these transcription factors.However,the molecular mechanisms under which Nanog regulates ES cell pluripotency are not very clear.P300 is a general coactivator in eukaryotes which usually helps transcription factors to regulate downstream genes.Nanog（or Nanog mutants） and its reporter gene were cotransfeted with p300 in cells in order to see if p300 could affect Nanog transcriptional activity.The results showed that p300 enhances Nanog transcriptional activity through the homeobox domain.