中文摘要：

Nanog 是它当白血病不在时维持 ES 房间的自强的能力识别的一个抄写因素禁止的因素(LIF ) 。Nanog 蛋白质包含 N 终端域(ND ) ， DNA 有约束力的 homeobox 域(HD ) 和 C 终端域(CD ) 。我们以前报导在 Nanog 的 CD 是 transcriptional 激活域必需品为在里面 Nanog 的 vivo 功能。这里，我们证明在 Nanog 的 ND 也是机能上地重要的。ND 的删除在人工的记者或本国的 Nanog 倡导者上减少 Nanog 的 transcriptional 活动。这截断的 Nanog 在调整内长的 Nanog 目标基因也是不太有效的。而且， ND 截断破坏了 Nanog 的能力也维持 ES 房间自强。我们发现 ND 没为 Nanog 的原子本地化被要求。这些结果建议象 oct3/4 和 rex-1 那样的内长的 pluripotent 基因的规定被要求为在里面 Nanog 的 vivo 功能。

英文摘要：

Nanog is a transcription factor identified by its ability to maintain the self-renewal of ES cells in the absence of leukemia inhibitory factor （LIF）. Nanog protein contains an N-terminal domain （ND）, a DNA-binding homeobox domain （liD） and a C-terminal domain （CD）. We previously reported that the CD in Nanog is a transcriptional activation domain essential for the in vivo function of Nanog. liere we demonstrated that the ND in Nanog is also functionally important. Deletion of the ND reduces the transcriptional activity of Nanog on either artificial reporters or native Nanog promoters. This truncated Nanog is also less effective in regulating the endogenous Nanog target genes. Furthermore, the ND truncation disrupted the ability of Nanog to maintain ES cell self-renewal as well. We found that the ND is not required for the nuclear localization of Nanog. These results suggest that the regulation of endogenous pluripotent genes such as oct3/4 and rex-1 is required for the in vivo function of Nanog.