研究了铝、氟及其交互作用对白茶和智仁早茶根际土壤酶的生态效应。结果表明,在单独施铝处理中,脲酶和多酚氧化酶活性受到抑制,其它土壤酶活性均随铝浓度的升高呈现先升后降的趋势,当Al浓度高达400mg/kg时,对酶呈现出抑制作用,影响达显著或极显著水平;在单独氟处理下,磷酸酶、尿酸酶活性变化不大,随着氟浓度的升高,除多酚氧化酶活性受抑制外,其余的酶活性也呈现出先升后降的趋势,当F浓度高达120 mg/kg时,对酶产生抑制作用,总体上氟的影响不如铝显著;铝、氟复合处理条件下,铝与氟之间联合作用对脲酶、蛋白酶、过氧化物酶、过氧化氢酶、多酚氧化酶、尿酸酶和抗坏血酸氧化酶活性产生了显著或极显著的交互效应,其作用方式因铝、氟处理浓度组合、酶及茶树品种的不同而不同。研究还发现,铝、氟对茶树根际土壤酶的交互效应与铝氟浓度的比值有密切关系,且不同的酶活性达最高时的铝氟比值基本相同,表明在茶树体内代谢中铝、氟之间存在一定的相关性。根际土壤酶活性在两个茶树品种间存在较大的基因型差异,尤其以酸性磷酸酶和抗坏血酸氧化酶最为显著,总体上白茶根际土壤酶对铝、氟及其交互作用较智仁早茶更敏感,两茶树品种之间土壤酶活性的差异与茶树根际过程密切相关。
The effects of A1 and F interaction on the soil enzyme of Bai tea and Zhirenzao tea rhizosphere were studied by means of pot culture experiments. Results revealed that Al significantly inhibited urease activity and polyphenoloxidase activity, and the activities of the other eight enzymes were increased significantly un- der low Al concentration (200 mg/kg), but decreased slightly under higher concentration (400 mg/kg). With the addition of F, polyphenoloxidase activity slightly decreased, and the activities of the others increased at low F concentration ( 40 mg/kg) and then decreased at higher F concentration (120 mg/kg), but there was no obvious effect on phosphatase or uric acidase. The effect of F on soil enzyme of tea rhizosphere was less significant than that of Al. When Al and F coexisted, interaction of Al and F showed different significant combination effects on enzyme activity, synergistic or antagonistic, which depending on the concentration association of Al and F, the specie of soil enzyme, as well as the variety of tea plant treated. We also found that with the coexistence of 40 mg/kg F and 200 mg/kg A1, almost all of the enzyme activities were stimulated and reached the highest point which indicating that some certain ratio of Al/F was needed for tea plant. In addition, obvious different response of soil enzyme to Al and F stress occurred between Bai tea and Zhirenzao tea rhizosphere, whether the difference had relation with the different tolerance to Al and F remain to probe into further.