中文摘要：

本研究主要探讨不同浓度NaCl胁迫处理下豇豆（Vigna unguiculata Linn.）幼苗叶片抗氧化酶活性的变化情况。研究结果表明,在0～250mmol/L NaCl胁迫下,随着盐浓度的增加,豇豆幼苗叶片可溶性蛋白质、脯氨酸和丙二醛含量逐渐增加,在150mmol/L浓度时,3者的含量都达到最大值;而超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性开始逐渐上升,它们的活性分别在100mmol/L、150mmol/L和150mmol/L时达到最大值,然后逐渐下降。同时,对NaCl胁迫下3种抗氧化酶基因的表达进行适时定量PCR分析,分析结果显示3种抗氧化酶基因的转录表达与酶活性的变化一致。说明在不同浓度的NaCl胁迫下,NaCl诱导了sod、pod和cat3种抗氧化酶基因的表达,3种抗氧化酶活性相应地提高,从而提高了豇豆应对NaCl胁迫的能力。本文结果将为今后豇豆在盐碱栽培生产提供一定的参考。

英文摘要：

This study focused on the changes of the antioxidant enzymes activities in cowpea seedlings（Vigna unguiculata L.） under different concentrative NaCl stress.The results showed that the content of soluble protein,proline and MDA in cowpea seedlings were gradually increased under 0～250 mmol/L NaCl stress,and their contents were maxmum at 150 mmol/L NaCl.However,the activities of superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT） were gradually increased and their activities were maximum at 100 mmol/L,150 mmol/L and 150 mmol/L,separately,and the activities falling thereafter.Meanwhile,quantitative real-time PCR analysis of expression of 3 antioxidase genes under NaCl stress also revealed that the over express of 3 antioxidant enzymes were induced by NaCl,and were consistent with the variance of their enzymic activities.Then the increasing of 3 enzymic activities enhanced the tolerance of cowpea against NaCl stress.The results of this paper would be provide a certain theoretical significance for cultivating and producing of cowpea in the future.