中文摘要：

以拟南芥cDNA为模板,用PCR扩增出GAPB的基因全长,然后将GAPB基因片段连接到PET28a（＋）载体上,构建重组质粒并转化大肠杆菌DH5α,经菌落PCR和酶切鉴定筛选出阳性克隆,测序正确后,再将阳性克隆的质粒转化大肠杆菌BL21（DE3）。结果表明：成功构建了原核表达载体PET28a（＋）-GAPB,为后续的GAPB融合蛋白的表达以及纯化奠定了基础。

英文摘要：

In this study,GAPB DNA fragment was amplified by polymerase chain reaction from Arabidopsis cDNA template.The GAPB DNA fragment was then connected into plasmid PET28a（＋）,and was transformed into E.coli DH5α.The positive clones were screened by colony PCR and enzyme digested,which were transformed into E.coli BL21 DE3 star plyss after sequencing.It was concluded that the prokaryotic expression plasmid PET28a（＋）GAPB was built successfully,which provided a material foundation for expression and purification of GAPB fusion protein.