中文摘要：

以转IrrE基因与非转基因甘蓝型油菜为材料，用10％（w／V）PEG6000（聚乙二醇）进行了干旱胁迫实验，比较研究了转IrrE基因与非转基因甘蓝型油菜幼苗的耐受性应答情况。结果表明：转基因油菜植株含水量下降趋势小于非转基因油菜植株；转基因油菜植株叶绿素含量和荧光参数F0的上升趋势均明显大于非转基因油菜植株；转基因油菜植株的Fv／Fn值和可溶性蛋白含量均大于非转基因油菜植株，但丙二醛（MDA）含量小于非转基因油菜植株。以上结果从侧面证实了：在干旱胁迫下，转IrrE基因油菜植株较非转基因植株有更强的耐受能力；，柑基因作为一种转录调节因子，广泛参与了油菜幼苗对干旱胁迫的耐受性应答过程，增强了植株的耐旱能力。

英文摘要：

The tolerant responses of transgenic-IrrE and non-transgenic Brassica napus L. under drought stress induced by 10% （W/V） PEG 6000 （polyethylene glycol） was comparatively studied. The physiological index such as relative water content （RWC）, chlorophyll content, chlorophyll fluorescence parameters Fo, Fv/Fm, the malondialdehyde （MDA） content and soluble protein contents were investigated. The results showed that the relative water content （RWC） and Fv/Fm of transgenic-lrrE and non-transgenic B. napus leaves decreased gradually, but the malondialdehyde （MDA） content, soluble protein contents, chlorophyll content and chlorophyll fluorescence parameters F0 all increased gradually. In a conclusion,transgenic-IrrE B. napus showed higher resistance to drought than non-transgenic B. napus. IrrE gene, as a transcription factor, participated in the processes of tolerance response to drought stress broadly, could increase the tolerance to drought stress in transgenic-IrrE B. napus.