基于已获得的控制小麦条锈病成株抗性“一致性”QTL区段80个SSR标记,结合小麦骨干亲本繁6及其衍生的39个后代小麦品种进行田间条锈病成株期抗性表型鉴定,揭示了骨干亲本繁6遗传物质及其成株抗性在其衍生品种的遗传规律。结果表明,骨干亲本繁6在条锈病条中31、32和33混合生理小种诱导下表现成株抗性,7个衍生后代品种表现全生育期抗性;用控制小麦条锈病成株抗性QTL区段的80个SSR标记对繁6及其后代衍生品种的其他亲本进行分子扫描,共发现9个来自繁6基因组的特异SSR标记,即Xwmc631、Xgwm359、Xwmc407、Xgwm501、Xgwm148、Xgwm539、Xgwm533、Xgwm299和Xgwm639,其中,Xwmc631、Xgwm359、Xgwm501、Xgwm299和Xgwm639在繁6衍生后代的4个子代中表现较高的遗传贡献率。以SSR标记与小麦条锈病成株抗性的关联分析发现6个SSR标记与小麦条锈病成株抗性显著相关,其中来自繁6的特异SSR等位变异Xgwm539—2D和Xgwm299-3B与严重度、反应型、普遍率、病情指数及病程曲线下面积(AUDPC)均具显著相关性,表明繁6的成株抗性及其控制遗传位点在其衍生后代品种选育过程中得到了很好的定向选择,并在西南麦区小麦条锈病抗性育种中发挥了重要作用。
Fan 6 is one of the most important founder parents of common wheat in China. The objective of this study was to reveal the genetic structure of Fan 6 and the transmission of its specific loci for adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici, Pst) in 39 derivatives. The resistance was evluated using 80 SSR markers assocated with the consensus QTLs for adult-plant resistance to Pst and the phenotypic assessment was conducted at seedling and adult stages after mixed inoculation with Pst races CYR31, CYR32, and CYR33. Fan 6 and seven derivatives showed resistance to Pst in the whole growth stage. Nine SSR allelles, Xwmc631, Xgwm359, Xwmc407, Xgwm501, Xgwm148, Xgwm539, Xgwm533, Xgwm299, and Xgwm639, were identified to be specific to Fan 6, of which Xwmc631, Xgwm359, Xgwm501, Xgwm299, and Xgwm639 exhibited high inheritability in the four derivate generations of Fan 6. The result of marker-trait association analysis showed that six SSR loci were significantly correlated with adult-plant resistance to Pst in Fan 6 and its derivatives. Among them, alleles from Fan 6 on loci Xgwm539 and Xgwm299 were responsible for disease severity, infection type, incidence, disease index, and area under the disease progress curve (AUDPC). These results indicated that the slow stripe-rusting resistance in Fan 6 has been intensively selected in breeding programs using Fan 6 as the founder parent, which will play an important role in Southwestern wheat area of China.