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塔里木裂腹鱼群体遗传多样性的AFLP分析
  • ISSN号:1000-6907
  • 期刊名称:淡水渔业
  • 时间:2012.7.7
  • 页码:83-87
  • 分类:S917.4[农业科学—水产科学] Q959.468.03[生物学—动物学]
  • 作者机构:[1]新疆维吾尔自治区水产科学研究所,乌鲁木齐830000
  • 相关基金:新疆维吾尔自治区自然科学基金项目(2009211A30);“国家科技基础条件平台一水产种质资源平台”项目;国家自然科学基金项目(31060356).
  • 相关项目:新疆塔里木河水系裂腹鱼类资源现状研究
中文摘要:

采用AFLP技术对喀拉喀什河、塔什库尔干河、阿克苏河多浪渠首、木扎提河4个群体共80尾塔里木裂腹鱼(Schizothorax biddulphi Günther)个体进行了遗传多样性分析。6对选择性扩增引物共扩增得到212个位点,其中多态性位点141个,多态位点比例为66.51%。4个群体的Shannon多样性指数(I)为0.316 9-0.544 3,Nei’s遗传多样性指数(H)为0.213 9-0.376 2,群体间的遗传距离为0.078 1-0.250 2。塔什库尔干河群体的多态位点比例、Shannon多样性指数和Nei’s遗传多样性指数均高于其它三个群体。AMOVA分析结果显示,群体总遗传变异85.17%来自群体内差异,而14.83%来自群体间差异,表明塔里木裂腹鱼遗传变异主要来源于群体内个体间,但群体间已存在一定程度的遗传分化。用UPGMA方法构建的群体系统进化树显示,多浪渠首群体和木扎提河群体首先聚类,然后依次与塔什库尔干河群体、喀拉喀什河群体聚类,这一方面与地理位置有关,另一方面与河流的自然环境有关。

英文摘要:

The genetic diversity of 80 Schizothorax biddulphi :individuals collected from Kalakashi River (K), Tashikuer- gan River (T), Duolang canal head of Akesu River (D) and Muzhati River (M) were analyzed by AFLP markers. A total of 212 loci were acquired by using 6 pairs of selective amplification primers, 141 of which were polymorphic, and the per- centage of polymorphic loci was 66. 51%. The Shannon diversity indices ranged from 0. 316 9 to 0. 544 3, the Neig gene diversity indices varied from 0. 213 9 to 0. 376 2, and the genetic distances among different populations were from 0. 078 1 to 0. 250 2. The percentage of polymorphic loci, Shannon diversity index, and Nei' s gene diversity index in Tashikuergan River population were higher than the corresponding values in other populations. The AMOVA results showed that variance within populations was 85.17% and among populations was 14. 83%. It suggested that the genetic variation occurred mainly within populations, but a certain degree of genetic differentiation existed among populations. The phylogeny tree constructed by UPGMA method revealed that the Duolang canal head population and Muzhati River population were first clustered to- gether, and then gathered with Tashikuergan population and Kalakashi River population respectively. The geographical lo- cations and river' s natural environment might be the main factors affecting the genetic diversity pattern of S. biddulphi.

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期刊信息
  • 《淡水渔业》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国水产学会 中国水产科学研究院长江水产研究所 中国水产科学研究院淡水渔业研究中心
  • 主编:陈大庆
  • 地址:湖北省武汉市东湖新技术开发区武大园一路8号
  • 邮编:430223
  • 邮箱:dsyy@chinajournal.net.cn
  • 电话:027-81780185
  • 国际标准刊号:ISSN:1000-6907
  • 国内统一刊号:ISSN:42-1138/S
  • 邮发代号:38-32
  • 获奖情况:
  • 中国自然科学核心期刊
  • 国内外数据库收录:
  • 中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:11619