中文摘要：

背景由于 proteomic 技术的快速的发展，最近，大进展在许多科学的地里被做了。我们试图使用基于的磁性的祷告(液体薄片)为肺腺癌( adCA )屏蔽特殊 biomarkers 的帮助矩阵的激光解吸附作用/电离 time-of-flight 团 spectrometry ( MALDI-TOF-MS )技术，并且交换磁性的祷告( MB-WCX )用弱阳离子建立诊断蛋白质 profiles.Methods 从 35 肺 adCA ， 46 良性的肺疾病( BLD )和 44 个健康个人的 sera 孤立并且净化低分子的重量蛋白质。锚 chip-MALDI-TOF-MS 获得的产生系列被 ClinProTools 和模式识别分析基因算法(GA ) 。在工作的团中的结果 800-10 变化 000 Da， 99 座特殊山峰在肺 adCA 对 BLD 被解决，当 101 座山峰在肺 adCA 对健康的人被解决时。能把 adCA 与 BLD 区分开来的 GA 获得的侧面与 80% 的敏感由 4053.88， 4209.57 和 3883.33 Da 组成， 93% 的特性当那能把 adCA 与健康控制分开时与 94% 的敏感由 2951.83 Da 和 4209.73 Da 组成， 95% 的特性。carcinoembryonic 抗原(CEA ) 在这个实验提供的敏感比我们的差别对待的侧面显著地低(P < 0.005 ) 。我们进一步识别了一个真核细胞的肽链版本因素 GTP 有约束力的子单元(eRF3b )(4209 Da ) 和可以为肺 adCA 用作候选人 biomarkers 的补充 C3f (1865 Da ) 。基于的 MALDI-TOF-MS 技术能很快并且有效地屏蔽的结论磁性的祷告从肺 adCA 病人和控制的 sera 的特殊蛋白质 / 多肽，它有潜力为为肺 adCA 建立一个新诊断方法珍视。

英文摘要：

Background Recently, due to the rapid development of proteomic techniques, great advance has been made in many scientific fields. We aimed to use magnetic beads （liquid chip） based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） technology to screen distinctive biomarkers for lung adenocarcinoma （adCA）, and to establish the diagnostic protein profiles. Methods Using weak cation exchange magnetic beads （MB-WCX） to isolate and purify low molecular weight proteins from sera of 35 lung adCA, 46 benign lung diseases （BLDs） and 44 healthy individuals. The resulting spectra gained by anchor chip-MALDI-TOF-MS were analyzed by ClinProTools and a pattern recognition genetic algorithm （GA）. Results In the working mass range of 800-10 000 Da, 99 distinctive peaks were resolved in lung adCA versus BLDs, while 101 peaks were resolved in lung adCA versus healthy persons. The profile gained by GA that could distinguish adCA from BLDs was comprised of 4053.88, 4209.57 and 3883.33 Da with sensitivity of 80%, specificity of 93%, while that could separate adCA from healthy control was comprised of 2951.83 Da and 4209.73 Da with sensitivity of 94%, specificity of 95%. The sensitivity provided by carcinoembryonic antigen （CEA） in this experiment was significantly lower than our discriminatory profiles （P 〈0.005）. We further identified a eukaryotic peptide chain release factor GTP-binding subunit （eRF3b） （4209 Da） and a complement C3f （1865 Da） that may serve as candidate biomarkers for lung adCA. Conclusion Magnetic beads based MALDI-TOF-MS technology can rapidly and effectively screen distinctive proteins/polypeptides from sera of lung adCA patients and controls, which has potential value for establishing a new diagnostic method for lung adCA.