中文摘要：

为了解黄颡鱼IgM基因表达的个体发生和IgM抗体代间传递机制,实验利用Real-time PCR和ELISA等技术研究了黄颡鱼IgM重链基因在卵巢、胚胎和仔鱼的表达变化,以及黄颡鱼IgM抗体在卵巢、胚胎和仔鱼中的含量变化。结果显示,黄颡鱼3-7 d仔鱼中,没有检测到IgM mRNA;14 d仔鱼IgM基因开始表达。用细菌免疫亲鱼后,对仔鱼IgM mRNA表达没有影响。黄颡鱼IgM抗体在卵巢、胚胎和仔鱼中都有分布,且呈现下降趋势,至9 d仔鱼中抗体水平最低（是卵抗体含量的0.31倍）。14 d仔鱼中IgM抗体水平上升（是9 d仔鱼抗体含量的1.6倍）。用细菌免疫亲鱼后,能显著提高胚胎、仔鱼中的IgM抗体水平,在卵中抗体含量提高了2.3倍,9 d仔鱼中提高了1.8倍。研究表明,黄颡鱼IgM抗体可以在母本和后代之间传递,早期仔鱼IgM抗体主要来自于亲本;因此,免疫亲鱼能显著增加子代的抗体水平。

英文摘要：

In order to understand the mechanism of development of IgM gene expression and its antibody transfer between generations in yellowcatfish,Pelteobagrus fulvidraco,IgM gene transcription change and its antibody level in ovary,eggs,embryos,and larvas of P. fulvidraco were investigated by using the Real-time PCR and ELISA techniques. Results were as follows： IgM mRNA not detected in 3-7 d larvae was found in 14 d larvae. Immuning mother fish by bacteria did not influence IgM mRNA expression level in their larvae. IgM antibody in P. fulvidraco exsited in eggs,embryos,and all early phases of larvae. These antibody expression level continued to decline and reached the lowest level in 9 d larvae（ which is 0. 31 times of eggs＇）,and began to increase in 14 d larvae（ which is 1. 6 times of 9 d larvae＇s）. Immuning mother fish by bacteria was able to significantly improve IgM antibody expression levels,which respectively increased to 2, 3 times and 1. 8 times in eggs and 9 d larvae of P. fulvidraco. These studies indicate that IgM gene expression of P. fulvidraco between generations is discontinuity,while its antibody expression is continous. The IgM antibody in early phases of larvae is from their mother fish. Immunizing may significantly improve antibody level of embryo and larvae.