中文摘要：

根据棉花GhCAD1基因的cDNA序列设计引物,采用PCR技术从棉花中克隆了GhCAD1基因的DNA序列,采用半定量RT-PCR方法研究GhCAD1基因在不同组织中的表达。结果表明：GhCAD1编码区DNA序列长度为2 898bp,包含5个外显子和4个内含子。分析发现这些内含子富含AT,在第1内含子中发现一个SBF-1因子结合位点。半定量RT-PCR检测表明,GhCAD1基因在不同组织中均有表达,其中叶部的表达量最高。GhCAD1表达量依次为叶〉铃壳〉苞叶〉花瓣〉根〉子叶。

英文摘要：

According to the cDNA sequence from cotton GhCAD1 gene,primers were designed.PCR method was used to clone GhCAD1 gene and the expression of GhCAD1 gene in different tissues of cotton was analyzed by semi-quantitative RT-PCR.A length of 2 898 bp sequence from genomic DNA of GhCAD1 was cloned by PCR.The genomic DNA of GhCAD1 contains five exons and four introns.Analysis of these introns indicated that they are rich in AT.Furthermore,a SBF-1 factor binding site was identified in the first intron of GhCAD1.Semi-quantitative RT-PCR analysis revealed that GhCAD1 can be expressed in different kinds of cotton tissues,and its mRNA accumulated most abundantly in leaf.GhCAD1 expression in cotton tissues was leafboll shellbractpetalrootcotyledon.