中文摘要：

目的 探讨不同剂量的硫酸镁（MgSO4）对大鼠脑源性肺损伤后神经源性肺水肿、血浆炎性因子TNF-α及肺组织病理形态学变化的影响.方法 将30只SD雄性大鼠按随机数字法分为假手术组（A组）、模型组（B组）及硫酸镁50 mg/kg干预组（C组）、硫酸镁100 mg/kg干预组（D组）、硫酸镁200 mg/kg干预组（E组）,每组6只.建立大鼠颅脑损伤模型后,硫酸镁干预组即刻按50 mg/kg 25％MgSO4腹腔注射,C组注射1次、D组注射2次、E组注射4次,每8h注射1次.A组及B组的大鼠注射相同剂量生理盐水作对照,C组大鼠注射1次及D组大鼠注射2次MgSO4后给予注射相同剂量的生理盐水作对照,注射方法及间隔时间同E组.伤后48 h测定大鼠肺组织含水量、血浆TNF-α浓度,肺组织常规HE染色,光镜观察肺组织病理形态学变化.结果 大鼠颅脑创伤后肺组织含水量均高于假手术组,以C组最明显（P＜0.05）,差异有统计学意义.B、C、D、E组大鼠之间肺组织含水量比较差异无统计学意义.B、C、D、E组大鼠TNF-q浓度均明显高于假手术组（P＜0.05）,D组血浆TNF-α浓度明显低于B组（P＜0.05）,E组血浆TNF-α浓度明显低于B组（P＜0.01）,其他各组间差异无统计学意义.假手术组肺组织形态正常,肺血管无扩张,无炎症细胞浸润;B、C、D、E组与假手术组比较均可见终末支气管腔内充满炎症细胞,周围肺组织的肺泡腔内可见炎细胞浸润,肺血管扩张、充血.B、C、D、E组在炎症细胞浸润及肺毛细血管扩张方面无明显差异.结论 脑外伤可导致脑源性肺损伤综合征,可导致神经源性肺水肿;硫酸镁可降低大鼠脑损伤后血浆TNF-α浓度,对肺水肿无明显影响.

英文摘要：

Objective To explore the different doses of magnesium sulfate on source sex rats neurogenic pulmona- ry edema after lung injury,plasma TNF-α inflammatory factors and the influence of the lung tissue pathology mor- phology. Methods 30 male SD rats by digital method were randomly divided into control group （group A） ,mod- el group （group B） and magnesium sulfate intervention group, magnesium sulfate intervention group is divided into 50 mg/kg （group C） ,100 mg/kg （group D） ,and 200 mg/kg group （group E） ,six in each group. Craniocerebral in- jury model was established, the rat made after the success of the mould, magnesium sulfate intervention group im- mediately by intraperitoneal injection of 50 mg/kg 25% MgSO4,group C injection once,twice group D and group E injection four times,getting a every 8 hours. Group A and group B the same dose of saline injection and injected one group C and group D injection after 2 times also injected the same dose of normal saline as control,group E with method. Determination of 48 hours after injury rat lung tissue water content, the plasma concentration of TNF-α, conventional HE staining, lung tissue were lung tissue pathology morphology. Results After craniocerebral trauma were lung tissue water content were higher than control group, the most obvious in group C （P〈0.05）, the difference was statistically significant. Group B,group C, D, E group there was no statistically significant difference compare between lung water content. B,C,D, E group of TNF-α concentrations were significantly higher than the control group （P〈0.05） ,plasma concentration of TNF-a D group was obviously lower than that of group B （P〈0.05） ,plasma concentration of TNF-α E group was obviously lower than that of group B （P〈0.01） ,there was no statistically significant difference between the other groups. Control form normal lung tissue, pulmonary vascular expansion, no inflammatory cell infiltration;B,C, D, E group compared with control group is visible terminal