中文摘要：

目的检测2型糖尿病患者及肥胖患者血浆颗粒蛋白前体（PGRN）水平，探讨其与慢性炎症及胰岛素抵抗的关系。方法80例2型糖尿病（T2DM）患者和88名正常糖耐量（NGT）个体以体重指数（BMI）t〉25kg／m2为切点分别分为正常体重（NW）亚组和肥胖（OB）亚组，检测空腹血糖（FPG）、糖负荷后2h血糖（2hPG）、HbA．、血脂、空腹胰岛素（FINS）水平，计算BMI和腰臀比，并以稳态模型评估胰岛素抵抗指数（HOMA．IR）及胰岛B细胞功能（HOMA．B）。采用ELISA法检测血浆PGRN及白细胞介素6（IL-6）水平。结果血浆PGRN水平在T2DM组高于NGT组（P〈0．01），在T2DM组和NGT组内，OB亚组PGRN水平高于对应NW亚组[（225．22±34．39对195．59±50．47和183．79±61．63对148．69±55．27）ng／m1．P〈0．05]，相关性分析提示血浆PGRN水平与体重、腰围、BMI、收缩压、FPG、2hPG、HbA、甘油三酯（TG）、IL-6、FINS、HOMA．IR呈正相关（P〈0．05或P〈0．01），与HOMA—B呈负相关（P〈0．05）。多元线性回归分析提示BMI、HbA。IL-6及TG是血浆PGRN水平的独立影响因素（P〈0．05）。结论2型糖尿病及肥胖患者血浆PGRN水平均升高，其升高程度与糖脂代谢紊乱、炎症及胰岛素抵抗的程度密切相关。

英文摘要：

Objective To detect plasma progranulin （PGRN）level in subjects with newly diagnosed type 2 diabetes mellitus and to investigate the relationship of plasma PGRN level with glycolipid metabolism, inflammation, and insulin resistance. Methods Eighty patients with newly diagnosed type 2 diabetes （T2DM） and 88 subjects with normal glucose tolerance （NGT） were recruited in the study. Both of them were divided into normal weight （NW） subgroup and obesity （OB） subgroup. Obesity was defined as body mass index （BMI）/〉 25 kg/m2 according to the World Health Organization-Western Pacific Region diagnostic criteria（ 2000 ）. Body fat parameters were measured and BMI, waist-to-hip ratio were determined. Fasting plasma PGRN and interleukin-6 （ IL-6 ） levels were determined by ELISA, fasting plasma glucose （ FPG）, 2 h plasma glucose after glucose loading （2hPG） , HbA, fasting insulin （FINS）, and lipids were also detected. Insulin resistance and pancreas 13 cell function were assessed by homeostasis model assessment （ HOMA-IR, HOMA-13）. Results Plasma PGRN level was significantly higher in T2DM group than that in NGT group（ P〈0.01 ）. Within groups of T2DM and NGT, plasma PGRN level in OB subgroups was higher than that in NW subgroups [ （ 225.22 ± 34.39 vs 195.59 ± 50.47 and 183.79 ± 61.63 vs 148.69 ± 55.27 ） ng/ml, P〈 0.05 ]. Bivariate correlation analysis showed that plasma PGRN level was positively correlated with weight, waist circumference, BMI, systolic blood pressure, FPG, 2hPG, HbAlc, triglyceride（TG）, IL-6, FINS, and HOMA-IR （ P〈0.05 or P〈0.01 ）, and was negatively correlated with HOMA-13 （ P〈0.05 ）. Multiple linear regression analysis showed that BMI, HbAlc, IL-6, and TG were independently related to plasma PGRN level（ P〈0.05 ）. Conclusions Plasma PGRN level was increased in patients with type 2 diabetes as well as in obesity, and was closely related with glyeolipid metabolism, inflammation, and insulin resistance.