中文摘要：

瞄准：在兔子和细胞 p53/c-Myc 蛋白质表示在植入的肝的 VX2 肿瘤生长的抑制上由静脉注射评估羟磷灰石 nano 粒子(Nano 幸运) 的效果。方法：60 只肝的 VX2 忍受肿瘤的兔子随机被划分成五个组。Nano 幸运溶胶 20 mg/kg， 40 mg/kg， 5-FU 答案 20 mg/mL， 5-FU 答案 20 mg/mL 的混合白酒和 Nano 幸运溶胶 20 mg/kg 被静脉灌输，作为控制进行的生理盐水。一般状态，重量，肝函数和粗野肿瘤体积动态地被检测。在肿瘤织物的 p53 和 c-Myc 基因蛋白质的表示被免疫组织化学方法检测。结果：植入的肝的 VX2 肿瘤的生长显著地在所有治疗组，被禁止在注射以后的 3 wk，肿瘤控制在 Nano 幸运溶胶组评价分别地是 25.5% 和 32.5% ，并且粗野肿瘤体积显然是不到控制的组。(24.81 +/- 5.17 和 22.73 +/- 4.23 对 33.32 +/- 5.26， P 【 0.05 ) 。5-FU 组的肿瘤控制率是 43.7%(18.74 +/- 4.40 对 33.32 +/- 5.26， P 【 0.05 ) ，但是在注射以后的动物的一般状态加重了；并且在药联合组的不利反应显然减少了。由于 Nano 幸运的效果，肿瘤的积极表达式联系了变异的 p53，在肿瘤织物的 c-Myc 与控制组相比显然被减少。结论：Nano 幸运在兔子上有明显的禁止的行动植入的肝的 VX2 肿瘤在活体内，它可以是减少的结果变异的 p53 和 c-myc 的表示，和药联合罐头显然减少 5-FU 的不利反应。

英文摘要：

AIM： To evaluate the effect of hydroxyapatite nano- particles （Nano HAP） by intravenous injection on the inhibition of implanted hepatic VX2 tumor growth in rabbits and cell p53/c-Myc protein expression. METHODS： 60 hepatic VX2 tumor-bearing rabbits was randomly divided into five groups. Nano HAP collosol 20 mglkg, 40 mg/kg, 5-FU solutions 20 mg/mL, mixed liquor of 5-FU solution 20 mg/mL and Nano HAP collosol 20 mg/kg were infused by vein, normal saline conducted as the control. The general state, weight, liver function and gross tumor volume were detected dynamically. The expression of p53 and c-Myc gene protein in tumor tissue was detected by immunohistochemistry methods. RESULTS： The growth of implanted hepatic VX2 tumors was significantly inhibited in all therapy groups, 3 wk after the injection, the tumor control rates in Nano HAP collosol groups were 25.5% and 32.5% respectively, and the gross tumor volumes were obviously less than that of control group. （24.81 ± 5.17 and 22.73 ± 4.23 vs 33.32 ± 5.26, P 〈 0.05）. The tumor control rate of 5-FU group was 43.7% （18.74 4± 4.40 vs 33.32 ± 5.26, P 〈 0.05）, but the general state of the animals after injection aggravated; and the adverse reaction in the drug combination group obviously decreased. Due to the effect of Nano HAP, the positive expression of tumor associated the mutated p53 and c-Myc in tumor tissue was decreased obviously compared with the control group. CONCLUSION： Nano HAP has evident inhibitory action on rabbit implanted hepatic VX2 tumor in vivo, which may be the result of decreasing the expression of the mutated p53 and c-myc, and drug combination can obviously decrease the adverse reaction of 5-FU.