中文摘要：

磷脂酶C（phospholipase C,PLC）是一类能够水解磷脂生成肌醇三磷酸与二酰甘油的酶,在植物非生物胁迫中起着重要的调控作用。本研究首次从大豆中克隆了大豆磷脂酶C基因Gm PLC12,其全长c DNA序列1 671 bp,编码一个由556个氨基酸组成的蛋白。系统发育树分析发现,Gm PLC12与菜豆Pv PLC1、豇豆Vu PLC同源性最近。利用实时定量PCR分析发现,Gm PLC12在盐、碱以及盐碱胁迫下表达量升高极为显著,尤其是盐碱胁迫。将Gm PLC12基因连接到原核表达载体中并利用IPTG诱导表达,产生一条约为70 k Da的条带,与预期结果一致。

英文摘要：

Phospholipase C （PLC） is a class of enzymes that cleave phospholipids into diacyl glycerol （DAG） and inositol 1,4,5-trisphosphate （IP3）. It plays an important role in plant abiotic stresses. In this study, we firstly cloned the GmPLC12 gene from soybean （Glycine max）. The full-length of GmPLC12 is 1 671 bp and encoding 556 amino acids. Phylogenetic analysis indicated that GmPLC12 shared highest homology with PvPLC1 and VuPLC. Real-time quantitative analysis showedthat the expression levels of GmPLC12 were induced by salt, alkali and salt-alkaline treatments, especially salt-alkaline stress. Subsequently, GmPLC12 was ligated into pE- T28a vector and transferred into Escherichia coli strain BL21. The recombinant protein was induced by IPTG and showed an about 70 kDa bands, which was consistent with prediction.