为了研究不同支链长度的3种环丙烯类乙烯作用抑制剂对寒富苹果冷藏后乙烯生物合成及果实衰老的影响,分别采用0.75μL/L的1-MCP(1-甲基环丙烯)、2μL/L的1-PentCP(1-戊基环丙烯)和0.5μL/L的1-OCP(1-辛基环丙烯)在常温(20±1)℃条件下,以不处理为对照,采用熏蒸处理寒富苹果20 h,然后装入0.02 mm厚度保鲜袋于低温(0±0.5)℃下贮藏。结果表明:与对照相比,1-MCP及其结构相似物处理均不同程度抑制了苹果冷藏期间的呼吸强度和乙烯释放量,推迟了呼吸峰和乙烯释放峰值出现的时间大约30 d;虽然1-MCP及其结构相似物处理在贮藏前期均促进了寒富苹果果实中ACS酶活性,导致处理果实在整个冷藏过程中ACC含量高于对照果实,但由于较好地抑制了ACO酶的活性,从而抑制了寒富苹果冷藏中乙烯的释放量;此外,3个处理还延缓了果实中MDA含量和细胞膜透性增大的速率。因此,1-MCP及其结构相似物处理能延缓冷藏中寒富苹果果实的成熟衰老进程,但1-MCP及其结构相似物处理间无显著差异。
Effect of treatment with 1-MCP or its structural analogues on ethylene synthesis and senescence of ‘Hanfu’apple during cold storage was investigated.After being fumigated for 20 h with 0.75 μL / L 1-MCP(1-methylcylopropene),2 μL / L 1-PentCP(1-pentylcyclopropene) or 0.5 μL / L 1-OCP(1-octylcyclopropene) respectively,the apple fruits were packed with 0.02 mm thick plastic bags and then stored at(0 ± 0.5) ℃.The results showed that treatment with 1-MCP or its structural analogues reduced ethylene production and respiration rate,delayed the respiration and ethylene peak by 30 d compared with control fruits.The content of ACC(1-aminocyclopropane-1-carboxylic acid) in treated fruits exhibited higher level than untreated control fruits throughout the whole cold storage,because treatment with 1-MCP or its structural analogues promoted the activity of ACS(ACC synthetase).All the treatments inhibited the activity of ACO(ACC oxidase),therefore,ethylene production of the treated fruits was suppressed during the cold storage.In addition,1-MCP and its structural analogue also deferred the increase in MDA content and membrane permeability,decreased respiration rate and ethylene production,and delayed senescence of ‘Hanfu’apple.However,there existed no significant differences between treatments with 1-MCP or its structural analogues.