目的考察多叶重楼 Paris polyphylla Smith var. polyphylla与云南重楼Paris polyphylla Smith var. yunnanensis(Franch.) Hand. -Mazz.中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的积累差异。方法HPLC法测定多叶重楼根、叶、茎中4种重楼皂苷含有量,建立20批样品的指纹图谱,聚类分析对2种植物不同部位中各重楼皂苷的积累差异进行评价。结果不同居群多叶重楼中各重楼皂苷含有量及其总量均有显著差异(P〈0.05,P〈0.01)。根中未检测到重楼皂苷Ⅵ,重楼皂苷Ⅰ、Ⅱ平均含有量最高;叶中重楼皂苷Ⅶ平均含有量和总含有量最高;茎中仅检测到重楼皂苷Ⅶ,总含有量最低。多叶重楼和云南重楼根的相似度较高,叶和茎较低。根据不同部位重楼皂苷总含有量,样品可分为3类。结论可考虑将多叶重楼作为云南重楼的替代品。
AIM To investigate the accumulation differences of polyphyllins Ⅰ , Ⅱ, Ⅵand Ⅶ in Paris polyphylla Smith var. polyphylla and Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz.METHODS The contents of four polyphyllins in the roots, leaves and stems of P. polyphyUa were determined by HPLC. The fingerprints of twenty batches of samples were established. Cluster analysis was adopted in the evalua- tion of accumulation differences of various polyphyllins in different parts of two kinds of plants. RESULTS There were significant differences in various polyphyllins' contents and total contents in different populations of P. polyphylla (P 〈 0.05, P 〈 0.01 ). Polyphyllin Ⅵ was not detected in the roots, and the average contents of polyphyllins Ⅰ and Ⅱ were the highest. The polyphyllin Ⅶ' s average content and total content were the highest in the leaves. Only polyphyllin Ⅶ was detected in the stems, and the total content was the lowest. The similarities of roots of P. polyphyUa and P. polyphylla vat. yunnanensis were high, while those of leaves and stems were low. According to the total contents of polyphyllins in different parts, the samples could be divided into three types. CONCLUSION P. polyphylla can be considered as the substitute of P. polyphylla var. yunnanensis.