中文摘要：

目的 探讨11种重楼属药用植物叶中主要次生代谢产物积累的差异。方法 采用超高效液相色谱法（UPLC）测定11种重楼属药用植物叶中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷的含量,建立其超高效液相色谱法图谱,并用单因素方差分析、色谱法分析对其叶中主要次生代谢产物积累差异进行研究。结果 11种重楼属药用植物叶中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量（P〈0.01）具有极显著差异,只有在阔瓣重楼、五指莲、黑籽重楼、长柱重楼和滇重楼叶中同时检测到重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ;11种重楼属药用植物叶超高效液相色谱法图谱差异较大,主要次生代谢产物种类也存在较大差异,其中阔瓣重楼、五指莲、黑籽重楼、长柱重楼和滇重楼叶超高效液相色谱法图谱相似度均在0.902以上,有16个共有峰,主要次生代谢产物种类差异较小。结论 11种重楼属药用植物叶合成重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ等主要次生代谢产物能力及种类存在较大差异,这可能是重楼属药用植物根茎中主要次生代谢产物含量及种类差异较大的主要原因。

英文摘要：

OBJECTIVE To investigate the differences of the accumulation of the main secondary metabolites in the leaves of 11 kinds of Paris L. medicinal plants. METHODS The contents of the main secondary metabolites, polyphyllin I , Ⅱ , Ⅵ, and Ⅷin the leaves of 11 kinds of Paris L. medicinal plants were determined by UPLC, and the UPLC fingerprints were established. The accu- mulation of the main secondary metabolites was evaluated by one-way ANOVA and chromatographic analysis. RESULTS There was significant difference（P 〈0. 01 ）in the contents of polyphyllin Ⅰ , Ⅱ , Ⅵ, and VII in the leaves of 11 kinds of Paris L. medicinal plants, and polyphyllin Ⅰ , Ⅱ , Ⅵ, Ⅶwere simutaneously detected only in vat. yunnanensis-1, P. axialis, P. thibetica, P. forrestii and var. yunnanensis ; there was significant difference in the UPLC chromatograms of 11 kinds of Paris L. medicinal plants, but the similarities among var. yunnanensis-1, P. axialis, P. thibetica, P. forrestii and var. yunnanensis all reached 0. 902 with 16 common peaks, indicating smaller difference in their main secondary metabolites. CONCLUSION There is significant difference in the abili- ties of the 11 kinds of Paris L. medicinal plants to accumulate polyphyllin Ⅰ, Ⅱ , Ⅵ, and Ⅶ , which may be the main reason that there are significant differences in the contents and classes of the major secondary metabolites of Paris L. roots.