中文摘要：

背景长期的妨碍的肺的疾病(COPD ) 与在航线的粘液生产过剩通常是复杂的。最近人的激活钙的氯化物隧道的增加的表示(CaCC1 ) 1 被发现在气喘的航线在粘液生产过剩起一个重要作用。在支气管的纸巾与 COPD， CaCC1 的表情， MUC5AC 和粘液在中国病人的航线调查 CaCC1 和粘液生产过剩的关系是 examined.Methods 支气管的纸巾在 2004 从 4 月在韦斯特中国医院里从 fiberoptic bronchoscopy 和支气管的活体检视被获得到 7 月。25 个病人与 COPD 生产过剩作为病人被诊断，并且另外的 20 是控制题目。在支气管的纸巾的 CaCC1 ， MUC5AC 和粘蛋白的表情被反向的transcriptase聚合酶链反应( RT-PCR )检测，在有 digoxigenin 的 situ 杂交(挖) -Iabeled RNA 探查， immunohistochemical 和 alcian 染色的蓝色周期的酸 Schiff (AB舞步)， respectively.Results 与控制组相比， CaCC1 的更强壮的表情进一步与 COPD ( P0.01 )从病人在整个支气管的纸巾被检测。而且， CaCC1 mRNA 的更强壮的表情与气流阻塞的严厉有关。从 COPD 的样品显示出在控制题目(P0.01 ) 的比那些的为 MUC5AC 的更强壮的染色，染色的 AB 舞步在 COPD 病人在控制题目(P0.01 ) 与那作比较的 submucosal 腺揭示了更多的粘蛋白。 CaCC1 mRNA 的表达式层次否定地分别地与病人一起被相关在/强迫了的一秒( FEV1 )强迫了吐气的体积重要能力( FVC )数据，FEV1%预言了数据，V50%预言了数据，V25%预言了数据( r=0.43 ， r=0.43 ， r=0.35 ， r=0.36 ， P0.01 ， P0.01 ， P0.05 ， P0.05 )。当 CaCC1 mRNA 的表示层次很好与 MUC5AC 的表示层次被相关时，航线上皮和 PAS-AB 的 mRNA 染色了 submucosal 腺的区域(r=0.39， r=0.46， P0.05， P0.01 ) 。MUC5AC mRNA 的表示层次否定地与病人的 FEV1/FVC 数据(P=0.01 ) 被相关， FEV1%pred 数据(P=0.01 ) ， V50% 预言了数据， V25% 预言了数据(r=0.53， r=0.53， r=0.48， r=0.43， P0.01， P0.01， P0

英文摘要：

Background Chronic obstructive pulmonary disease （COPD） is usually complicated with mucus overproduction in airway. Recently the increased expression of the human calcium-activated chloride channel 1 （CaCC1） was found to play an important role in mucus overproduction in the asthmatic airways. To investigate the relationship of CaCC1 and mucus overproduction in the airway of Chinese patients with COPD, the expressions of CaCC1, MUC5AC and mucus in bronchial tissues were examined. Methods Bronchial tissues were obtained from fiberoptic bronchoscopy and bronchial biopsy in West China Hospital from April to July in 2004. Twenty-five patients were diagnosed as the patients with COPD overproduction, and other 20 were the control subjects. The expressions of CaCC1, MUC5AC and mucin in bronchial tissues were detected by reverse transcriptase-polymerase chain reaction （RT-PCR）, in situ hybridization with digoxigenin （DIG）-Iabeled RNA probe, immunohistochemical and alcian blue-periodic acid Schiff （AB-PAS） staining, respectively. Results Compared with the control group, the stronger expressions of CaCC1 were further detected throughout the bronchial tissues from patients with COPD （P〈0.01）. Furthermore, the stronger expressions of the CaCC1 mRNA were related to the severity of airflow obstruction. Samples from COPD showed a stronger staining for MUC5AC than those in control subjects （P〈0.01） and AB-PAS staining revealed more mucins in COPD patients＇ submucosal gland comparing with that in control subjects （P〈0.01）. Expression levels of the CaCC1 mRNA were respectively negatively correlated with the patients＇ forced expiratory volume in one second （FEV~） / forced vital capacity （FVC） data, FEV1% predicted data, V50% predicted data, V25% predicted data （r=-0.43, r=-0.43, r=-0.35, r=-0.36, P〈0.01, P〈0.01, P〈0.05, P〈0.05）. While the expression levels of the CaCC1 mRNA were well correlated with the expression levels of the MUC5AC mRNA of airway epithelium and the