中文摘要：

目的丙烯醛雾化吸入2周诱导小鼠气道重塑模型,研究NF-κB易位抑制剂咖啡酸苯乙酸酯（CAPE）对小鼠早期气道重塑的干预作用。方法24只C57BL/6小鼠随机分为4组（每组6只）正常对照组、单纯CAPE组（腹腔内注射CAPE30mg/kg,隔日一次）、丙烯醛组（丙烯醛雾化吸入2h×3次/d,共2周）、丙烯醛雾化合并CAPE干预组（雾化吸入丙烯醛同时腹腔内注射CAPE）。于实验第2周采取各组小鼠肺组织和支气管肺泡灌洗液（BALF）。肺组织HE、MASSON染色和羟脯氨酸定量观察气道平滑肌增生、气道上皮下及管周肺组织胶原沉积情况,Ashcroft评分计算HE染色下小鼠气道及管周肺组织的纤维化严重程度;BALF行细胞计数观察气道炎症反应,ELISA测定BALF中TGF-β1含量。结果（1）丙烯醛组气道炎症反应,平滑肌增生及气道上皮下胶原沉积较正常对照组显著增高（P〈0.05）。（2）丙烯醛雾化合并CAPE干预组气道炎症反应及管周肺组织平滑肌增生,上皮下基底膜沉积较丙烯醛组明显减轻（P〈0.05）。结论NF-κB抑制剂CAPE能够减少致纤维化因子TGF-β1的释放,减轻早期气道炎症反应及重塑的进程。

英文摘要：

Objective： To investigate the effect of caffeic acid phenethyl ester （CAPE）, an inhibitor of NF - κB on early airway remodeling of mice inhaled with acrolein fog for two weeks. Methods ： Early airway remodeling model of mice was established with acrolein inhalation 6 h per day for two weeks.Twenty - four C57BL/6 mice were randomly divided into four groups （n = 6 in each group） . Mice with or without acmlein inlation were intraperitonealy injected with CAPE 30 mg/kg qod, and saline was administered in control mice. Lung tissue sections were stained with HE, Masson and evaluated with Ashcroft scot, s. Hydroxyproline concentration of lung tissues was detected.Total and differential cell count and TGF-β1 concentration in BALF were carried out by micrescopy and ELISA, respectively. Results： Comparing with control, increased airway inflammation, smooth muscle cells proliferation and sub - epithelial collagen deposition were observed in mice inhaled with acrolein fog （ P 〈 0.05）, which was significandy reduced after the treatment with CAPE （ P 〈 0.05） . Accordingly, the increased release of TGF-β1 in BALF from mice inhaled with acmlein was attenuated with CAPE. Conclusion： Through decreased release of TGF- β1, NF- κB inhibitor CAPE inhibits acrelein- induced inflammation and development in early airway remodeling.