欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的探讨脂多糖（Lipopolysaccharide，LPS）诱导人肺泡上皮细胞（A549）一氧化氮（Nitric oxide，NO）分泌的影响。方法对A549给予不同浓度的LPS，分别收集其培养上清液，用硝酸还原法检测NO浓度。结果不同浓度LPS诱导A549，NO分泌量高于未经LPS诱导的对照组，且具有浓度、时间依赖性。结论LPS诱导A549NO的分泌，提示LPS具有诱导肺泡上皮细胞急性炎症和氧化应激的作用。
英文摘要：
Objective To investigate the effects of quercetin on nitric oxide （NO） in human pulmonary epithelial cells （A549） induced by lipopolysaccharide （LPS）. Methods The supernates of LPS-induced cultured A549 from different intervention groups were collected to measure the concentration of NO by nitrate reductase assay. Results The concentration of NO in supernates of LPS-induced A549 was higher than that in the control group （P〈0. 05） in a dose- and time-dependent manner. The concentration of NO in supernates of quercetin intervention groups at 10, 15μmol/L was lower than that in non-intervention group （P〈0. 01）, but that at 5μmol/L was no difference （P〉 0.05）. Conclusion Quercetin can decrease LPS-induced NO secretions in A549, which suggests that quercetin has anti- inflammatory and anti-oxidant stress properties on A549.
同期刊论文项目
同项目期刊论文
Decreased expression of human aquaporin-5 correlated with mucus over production in airways of chroni
Ghrelin inhibits interleukin-8 production induced by hydrogen peroxide in A549 cells via NF-kappaB p
Peroxisome proliferator-activated receptor γ inhibits acrolein-induced airway mucus hypersecretion i
Increased expression of Human calcium-activated chloride channel 1 gene is correlated with mucus ove
Aminoguanidine attenuates bleomycin-induced pulmonary fibrosis in rats through in hibition of heat s
Ghrelin inhibit cell apoptosis in pancreatic beta cell line HIT-T15 via mitogen-activated protein ki
期刊信息
位置：