中文摘要：

目的：探讨CD98与炎症性肠病（ IBD）的关系。方法：雄性Balb/c小鼠48只，分为4组。正常对照组：不进行干预，自由饮食。生理盐水组：灌肠前禁食24 h，自由饮水，于实验第1、8及15天分别用100μL的生理盐水灌肠，灌肠后自由饮食。乙醇组：用体积分数50％乙醇溶液100μL灌肠，方法和步骤同生理盐水组。 TNBS＋乙醇组：第1、8及15天分别用2．0、2．5和3．0 mg TNBS加体积分数50％乙醇溶液100μL灌肠，建立TNBS诱发的小鼠实验性IBD模型。实验第21天，采用流式细胞仪检测外周血CD98的表达，免疫组化SABC法检测结肠黏膜上皮组织中CD98蛋白的表达。结果：4组小鼠外周血和结肠黏膜上皮组织中CD98蛋白表达的差异均有统计学意义（F＝29．460，χ2＝4．567，P＜0．05），TNBS＋乙醇组高于其他3组。结论：CD98可能参与了IBD的发生发展。

英文摘要：

Aim：To explore the pathogenic role of CD98 in inflammatory bowel disease（IBD）.Methods：The IBD model in mice was induced by TNBS .Forty-eight male Balb/c mice were randomly divided into four groups （n=12）.Nor-mal control group：mice were given an unrestricted diet .Normal saline group： mice were put in a fasted state for twenty-four hours,but could drink water free , then given unrestricted diet after having received a 100 μL normal saline enema on the first,eighth,and fifteenth day of the experiment .Ethanol group：mice were given 100 μL 50%solution ethanol enema , other steps were the same to the normal saline group .TNBS＋ethanol group：mice were respectively instilled by 2.0,2.5, and 3.0 mg TNBS dissolved in 100 μL 50%ethanol enema on the first , eighth,and fifteenth day of the experiment .The CD98 in peripheral blood and colonic epithelial tissue were detected by flow cytometry and immunohistochemistry ,respec-tively.Results：The expression of CD98 in peripheral blood and colonic epithelial tissue significantly increased in the TN-BS＋ethanol group as compared with the other three groups （F=29.460,χ2 =4.567,P〈0.05）.Conclusion：CD98 may be involved in the pathogenesis of IBD .