中文摘要：

目的 探讨经内镜逆行胰胆管造影术（endoscopic retrograde cholangiopancreatography,ERCP）治疗中对十二指肠镜进行重复高水平消毒的效果及对术后胆管炎发生率的影响。方法 本研究选取郑州大学第二附属医院收治的721例ERCP术患者进行研究,选取时段为2013年6月—2015年12月期间,采用随机数字法将其分为2组,其中常规组354例,使用常规消毒的十二指肠镜;重复消毒组367例,使用重复高水平消毒的十二指肠镜。比较2组内镜消毒效果及患者术后胆管炎的发病率。结果 重复消毒组内腔菌落计数（8.6±9.1）cfu/件、抬钳器菌落计数（10.0±4.8）cfu/件,常规消毒组内腔菌落计数（12.6±8.2）cfu/件、抬钳器菌落计数（15.1±8.2）cfu/件,重复消毒组十二指肠镜内腔冲洗液和抬钳器棉拭子培养的菌落计数显著高于常规消毒组十二指肠镜（P〈0.05）。重复消毒组患者术后胆管炎的发病率为2.23%,显著低于常规消毒组的5.36%（P〈0.05）。而2组患者术中接受操作种类、合格率、镜头上菌落比较差异无统计学意义（P〉0.05）。结论 在ERCP术治疗中对十二指肠镜进行重复高水平消毒具有明显的效果,可显著降低ERCP术后胆管炎的发病率,在临床上值得大范围推广和应用。

英文摘要：

Objective Study the treatment effects of ERCP in the duodenoscopy with repeated high-quality disinfection, as well as its influence on the incidence of postoperative cholangitis. Methods This study selected 721 cases of from ERCP operations in our hospital, ranging from June, 2013 to December, 2015, and randomly divided into two groups, including normal group with 354 cases, using duodenoscopy with conventional disinfection and repeated disinfection group with 367 cases, using duodenoscopy with the repeated high-quality disinfection. Compare two groups of patients with endoscopic dis- infection effect and the incidence of postoperative cholangitis. Results Repeated disinfection group lumen colony count （8.6 ± 9.1 ） cfu/parts, lifting clamp colony count （10.0 ± 4.8 ） cfu/parts ,routine disinfection group lumen colony count （ 12.6 ± 8.2） cfu/parts,lifting clamp colony count （ 15.1 ± 8.2） cfu/parts, repeated disinfection within the group of duo- denoscopy cavity flushing fluid and a cotton swab cultivate up pliers colony count group is significantly higher than the conventional disinfection duodenoscope （ P 〈 0. 05 ）. For repeated disinfection patients the incidence of postoperative cholangitis was 2.23 %, significantly less than 5.36% of the conventional disinfection group （P 〈 0.05 ）. And there were no statistical difference between the two groups in operative types, the qualification rates and lens colony （ P 〉 0.05 ）. Conclusion In ERCP treatment of duodenoscopy repeated high-qulaity disinfection has obvious effect, which can signifi- cantly reduce the incidence of postoperative cholangitis ERCP, worthy of widespread popularization and application in clinic.