中文摘要：

类泛素的小蛋白修饰（small ubiquitin-like modifier,SUMO）对植物抗病防御起重要作用。本研究利用生物信息学方法,确定了大豆SUMO系统6个相关基因。对细菌性斑点病抗病品种黑农37及感病品种合丰25接种处理后各基因表达变化进行实时定量分析。结果发现：抗病品种中,GmSUMO2、GmSCEa在叶中24h表达量最高,GmSUMO3、GmSCEb在叶中48h表达量最高,所以GmSUMO2/3和GmSCEa/b在大豆叶部抗病过程中起重要作用。GmSAE2a在茎中48h的表达量高达6倍多,在茎部抗病过程中起主要作用。GmESD4e在抗病品种的根和叶中表达量快速下降,起着主要的去SUMO化作用。GmESD4a/e在感病品种接菌前期和后期分别在叶和根部相对表达量较高,主导感病品种叶和根部的去SUMO化作用,初步说明大豆SUMO系统与细菌性斑点病抗性相关。

英文摘要：

Sumoylation played an important role in pathogen defense of soybean. By bioinformatics method,6SUMO related genes were obtained from soybean. Expression of these genes were analyzed at transcriptional level by real- time PCR after inoculated with Pseudomonas syringae pv. glycinea in resistant variety HN37（ Heinong37） and susceptive variety HF25（ Hefeng 25）. Results showed that,in HN37 leaf,expressions of GmSUMO2 and GmSCEa were the highest at 24 h,GmSUMO3 and GmSCEb were at 48 h. Therefore GmSUMO2 /3 and GmSCEa / b might have played a major role in leaf disease- resistance process. Expression of GmSAE2 a at 48 h was 6- fold higher than at 0h which might have played a major role in stem disease- resistance. Expression of GmESD4 e decreased significantly after inoculation,which might play a major role of desumoylation in HN37. Expression of GmESD4 a / e was the highest at 24 h in leaf and at 72 h in root of HF25 which might have played a major role of desumoylation of HF25.In summary,the soybean SUMO system was associated with resistance to P. syringae pv. glycinea.