中文摘要：

从大豆品种天隆1号的叶片中克隆GmNup96基因的cDNA序列，对其编码的氨基酸序列、蛋白质理化性质、一级结构、二级结构、亚细胞定位等进行了生物信息学分析。结果表明：GmNup96基因编码1022个氨基酸，为具有一定亲水能力的酸性蛋白，不具有信号肽，相对分子量为116．1997kDa；二级结构预测结果显示，GmNup96序列存在α-螺旋（46．87％）、无规则卷曲（26．32％）、延伸链（17．03％）和β-转角（9．78％），并无其它二级结构；系统进化树分析表明，大豆GmNup96基因与野生大豆、芸豆、绿豆、红小豆之间的亲缘关系更近。

英文摘要：

The cDNA of GmNup96 gene in Tianlong 1 was cloned, and its bioinformatics character was analyzed. The results showed that the gene encoded a 116. 199 7 kDa protein with 1 022 amino acids , which was a hydrophilic protein without signal peptide, and the GmNup96 protein contained a conserved autoproteolytic peptidase domain in the N terminus. Through the secondary structure prediction, GmNup96 protein contained alpha-helix （46. 87% ）, beta turn （ 9. 78% ）, extended strand （ 17.03% ） and random coil （26. 32% ）. Phylogenetic analysis of GmNup96 and its homologs with different plant species were conducted by the software MEGA 5.1. The results showed that GmNup96 from Glycine max were near with. Glycine soja, Phaseus vulgaris, Vigna radiata var. radinta and Vigna angularis in genetic relationship.