中文摘要：

为探讨SUMO（类泛素的小蛋白修饰,small ubiquitin-like modifier）在大豆逆境胁迫中的作用,对大豆SUMO系统的相关基因和蛋白质结构进行分析,与其它植物SUMO化相关基因构建系统发育树,并将大豆合丰25热激处理后,选取SUMO系统中的6个基因（Gm SUMO2,Gm SUMO3,Gm SAE1b,Gm SCEb,Gm E3f,Gm ESD4d）进行实时定量分析。结果表明,大豆Gm SUMO,Gm SAE1,Gm SCE,Gm E3f与木本植物亲缘关系较近;热激处理不同时段,大豆6个SUMO相关基因均有明显变化,Gm SAE1b起激活作用最先启动,Gm SUMO2/3和Gm SCEb表达趋势一致,验证了Gm SCEb的结合作用。热激10min后,Gm SUMO2/SUMO3相对表达量达到最低,而Gm ESD4d在叶中相对表达量达到近40倍,说明Gm ESD4d在叶中起去SUMO化作用。热激30min,在根中Gm SUMO2相对表达量达到近27倍,Gm SCEb达到10倍,Gm E3f达到近40倍。说明Gm SUMO2、Gm SCEb、Gm E3f主要在根中起作用。

英文摘要：

SUMO is an ubiquitin like peptide that binds to the target protein after translation. The sumoylation in plant has demonstrated an important role for the process in growth and development,stress response such as heat shock,flowering control and pathogen defense. In this study,soybean SUMO related gene and protein structure were analyzed by bioinformatics tools. The phylogenetic tree showed that most of soybean SUMO related genes were closely related to woody plants,which indicated that soybean SUMO related genes（Gm SUMO,Gm SAE1,Gm SCE,Gm E3f） evolved from woody plant. Six genes in SUMO（Gm SUMO2,Gm SUMO3,Gm SAE1 b,Gm SCEb,Gm E3 f,and Gm ESD4d） were analyzed by real-time PCR. With 0 to 30 min heat shock treatments,the gene expression of6 soybean SUMO genes showed obvious change,Gm SAE1 b first starts. After 10 min,relative expression level of Gm SUMO2/3 reached the minimum. However,relative expression level of Gm ESD4 d raised nearly 40 fold in leaf,which might play a major role of desumoylation in leaf. Then,30 min after heat shock,relative expression level of Gm SUMO2 increased nearly 27 times,Gm SCEb 10 times,Gm E3 f nearly 40 times in root,which showed that GmSUMO2,Gm SCEb and Gm E3 f mainly functioned in the root.